In vitro investigation of Debaryomyces hansenii strains for potential probiotic properties
- 504 Downloads
In this study, 23 Debaryomyces hansenii strains, isolated from cheese and fish gut, were investigated in vitro for potential probiotic properties i.e. (1) survival under in vitro GI (gastrointestinal) conditions with different oxygen levels, (2) adhesion to Caco-2 intestinal epithelial cells and mucin, and (3) modulation of pro- and anti-inflammatory cytokine secretion by human monocyte-derived dendritic cells. As references two commercially available probiotic Saccharomyces cerevisiae var. boulardii (S. boulardii) strains were included in the study. Our results demonstrate that the different D. hansenii yeast strains had very diverse properties which could potentially lead to different probiotic effects. One strain of D. hansenii (DI 09) was capable of surviving GI stress conditions, although not to the same degree as the S. boulardii strains. This DI 09 strain, however, adhered more strongly to Caco-2 cells and mucin than the S. boulardii strains. Additionally, two D. hansenii strains (DI 10 and DI 15) elicited a higher IL-10/IL-12 ratio than the S. boulardii strains, indicating a higher anti-inflammatory effects on human dendritic cells. Finally, one strain of D. hansenii (DI 02) was evaluated as the best probiotic candidate because of its outstanding ability to survive the GI stresses, to adhere to Caco-2 cells and mucin and to induce a high IL-10/IL-12 ratio. In conclusion, this study shows that strains of D. hansenii may offer promising probiotic traits relevant for further study.
KeywordsAdhesion In vitro gastrointestinal screening Immunomodulation Yeasts
The research leading to these results was funded by the EU’s Seventh Framework Programme (FP7) under Grant agreement PITN-GA-2010-264717 for the Cornucopia project. The authors would like to thank late Professor Jure Piškur for taking initiative to the establishment of the project and Teun Boekhout at Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands for the yeast strains CBS 5307 and CBS 8339.
Compliance with ethical standards
Conflict of interest
The authors declare that there are no conflicts of interest.
- Andlid T, Vázquez-Juárez R, Gustafsson L (1998) Yeasts isolated from the intestine of rainbow trout adhere to and grow in intestinal mucus. Mol Mar Biol Biotechnol 7:115–126Google Scholar
- Andreoletti O, Budka H, Buncic S et al (2008) The maintenance of the list of QPS microorganisms intentionally added to scientific opinion of the panel on biological hazards adopted on 10 December 2008. EFSA J 923:1–48Google Scholar
- Dancey CP, Reidy J (2004) Statistics without maths for psychology: using SPSS for windows, 3rd edn. Pearson Education Limited, LondonGoogle Scholar
- Dunne C, O’Mahony L, Murphy L et al (2001) In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings. Am J Clin Nutr 73:386S–392SGoogle Scholar
- FAO (2006) Probiotics in food. FAO Food Nutr Pap 85:1–21Google Scholar
- Harish K, Varghese T (2006) Probiotics in humans—evidence based review. Calicut Med J 4:1–11Google Scholar
- Isolauri E (2001) Probiotics in human disease. Am J Clin Nutr 73:1142S–1146SGoogle Scholar
- Maccaferri S, Klinder A, Brigidi P et al (2012) Potential probiotic Kluyveromyces marxianus B0399 modulates the immune response in Caco-2 cells and peripheral blood mononuclear cells and impacts the human gut microbiota in an in vitro colonic model system. Appl Environ Microbiol 78:956–964. doi: 10.1128/AEM.06385-11 CrossRefGoogle Scholar