Genetic dissection of acetic acid tolerance in Saccharomyces cerevisiae
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Dissection of the hereditary architecture underlying Saccharomyces cerevisiae tolerance to acetic acid is essential for ethanol fermentation. In this work, a genomics approach was used to dissect hereditary variations in acetic acid tolerance between two phenotypically different strains. A total of 160 segregants derived from these two strains were obtained. Phenotypic analysis indicated that the acetic acid tolerance displayed a normal distribution in these segregants, and suggested that the acetic acid tolerant traits were controlled by multiple quantitative trait loci (QTLs). Thus, 220 SSR markers covering the whole genome were used to detect QTLs of acetic acid tolerant traits. As a result, three QTLs were located on chromosomes 9, 12, and 16, respectively, which explained 38.8–65.9 % of the range of phenotypic variation. Furthermore, twelve genes of the candidates fell into the three QTL regions by integrating the QTL analysis with candidates of acetic acid tolerant genes. These results provided a novel avenue to obtain more robust strains.
KeywordsAcetic acid tolerance Phenotypic analysis QTL Saccharomyces cerevisiae SSR markers
This work was supported by the Natural Science Foundation of Jiangsu Province (Grant No. BK2012363) and the Outstanding Youth Foundation of Jiangsu Province (Grant No. BK20140002).
- Bretagne S, Costa JM, Besmond C, Carsique R, Calderone R (1997) Microsatellite polymorphism in the promoter sequence of the elongation factor 3 gene of Candida albicans as the basis for a typing system. J Clin Microbiol 35:1777–1780Google Scholar
- Holyoak CD, Bracey D, Piper PW, Kuchler K, Coote PJ (1999) The Saccharomyces cerevisiae weak-acid-inducible ABC transporter Pdr12 transports fluorescein and preservative anions from the cytosol by an energy-dependent mechanism. J Bacteriol 181:4644–4652Google Scholar
- Kawahata M, Masaki K, Fujii T, Iefuji H (2006) Yeast genes involved in response to lactic acid and acetic acid: acidic conditions caused by the organic acids in Saccharomyces cerevisiae cultures induce expression of intracellular metal metabolism genes regulated by Aft1p. FEMS Yeast Res 6:924–936CrossRefGoogle Scholar
- McCusker JH, Clemons KV, Stevens DA, Davis RW (1994) Genetic characterization of pathogenic Saccharomyces cerevisiae isolates. Genetics 136:1261–1269Google Scholar
- Schlötterer C, Wiehe T (1999) Microsatellites, a neutral marker to infer selective sweeps. In: Goldstein DB, Schlötterer C (eds) Microsatellites-evolution and applications. Oxford University Press, Oxford, pp 238–248Google Scholar
- Tenreiro S, Rosa PC, Viegas CA, Sá-Correia I (2000) Expression of the AZR1 gene (ORF YGR224w), encoding a plasma membrane transporter of the major facilitator superfamily, is required for adaptation to acetic acid and resistance to azoles in Saccharomyces cerevisiae. Yeast 16:1469–1481CrossRefGoogle Scholar