Physiological and proteomic analysis of salinity tolerance of the halotolerant cyanobacterium Anabaena sp

  • Ravindra Kumar Yadav
  • Preeti Thagela
  • Keshawanand Tripathi
  • G. Abraham
Original Paper
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Abstract

The halotolerant cyanobacterium Anabaena sp was grown under NaCl concentration of 0, 170 and 515 mM and physiological and proteomic analysis was performed. At 515 mM NaCl the cyanobacterium showed reduced photosynthetic activities and significant increase in soluble sugar content, proline and SOD activity. On the other hand Anabaena sp grown at 170 mM NaCl showed optimal growth, photosynthetic activities and comparatively low soluble sugar content, proline accumulation and SOD activity. The intracellular Na+ content of the cells increased both at 170 and 515 mM NaCl. In contrast, the K+ content of the cyanobacterium Anabaena sp remained stable in response to growth at identical concentration of NaCl. While cells grown at 170 mM NaCl showed highest intracellular K+/Na+ ratio, salinity level of 515 mM NaCl resulted in reduced ratio of K+/Na+. Proteomic analysis revealed 50 salt-responsive proteins in the cyanobacterium Anabaena sp under salt treatment compared with control. Ten protein spots were subjected to MALDI-TOF–MS/MS analysis and the identified proteins are involved in photosynthesis, protein folding, cell organization and energy metabolism. Differential expression of proteins related to photosynthesis, energy metabolism was observed in Anabaena sp grown at 170 mM NaCl. At 170 mM NaCl increased expression of photosynthesis related proteins and effective osmotic adjustment through increased antioxidant enzymes and modulation of intracellular ions contributed to better salinity tolerance and optimal growth. On the contrary, increased intracellular Na+ content coupled with down regulation of photosynthetic and energy related proteins resulted in reduced growth at 515 mM NaCl. Therefore reduced growth at 515 mM NaCl could be due to accumulation of Na+ ions and requirement to maintain higher organic osmolytes and antioxidants which is energy intensive. The results thus show that the basis of salt tolerance is different when the halotolerant cyanobacterium Anabaena sp is grown under low and high salinity levels.

Keywords

Anabaena sp Cyanobacterium Growth Osmotic adjustment Proteomics Salinity Survival 
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Notes

Acknowledgments

The work was conducted as part of the in-house research project of GA. We are grateful to Indian Agricultural Research Institute, New Delhi and the ICAR-Indian Council for Agricultural Research for financial assistance, facilities and encouragement.

Author contribution

GA planned the experiments and provided intellectual in puts. RKY helped in conducting the 2-D gel electrophoresis related work and recording the data. PT helped in the proteomic work and soft ware analysis. K.N.T. performed the experiments related to PS I, PS II and whole chain activity.

Compliance with ethical standards

Conflict of interest

We declare that there is no conflict of interest.

Supplementary material

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Supplementary material 1 (DOC 125 kb)
11274_2016_2098_MOESM2_ESM.docx (3.6 mb)
Supplementary material 2 (DOCX 3650 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Ravindra Kumar Yadav
    • 1
  • Preeti Thagela
    • 1
  • Keshawanand Tripathi
    • 1
  • G. Abraham
    • 1
  1. 1.Centre for Conservation and Utilization of BGAICAR-Indian Agricultural Research InstituteNew DelhiIndia

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