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Anti-biofouling property studies on carboxyl-modified multi-walled carbon nanotubes filled PDMS nanocomposites

  • Yuan Sun
  • Zhizhou ZhangEmail author
Original Paper

Abstract

Polydimethylsiloxane (PDMS) with exceptional fouling-release properties is extremely susceptible to the microfouling resulted from the colonization of the pioneer microorganisms in the marine environment. In this study, six carboxyl-modified multi-walled carbon nanotubes (cMWNTs) nanoparticles were incorporated into the PDMS matrix, respectively, in order to produce the cMWNTs-filled PDMS nanocomposites (CPs) with improved antifouling (AF) properties. The AF properties of the six CPs were examined via the field assays conducted in Weihai, China. The effects of the anti-biofouling potential of the CPs (i.e. the P3 surface) on the colonization of the pioneer prokaryotic and eukaryotic microbes were investigated using the single-stranded conformation polymorphism technique via the comparison of the diversity indices. Different CPs have displayed differential and better AF properties as compared to that of the unfilled PDMS (P0). The P3 surface has exhibited exceptional anti-biofouling capacity compared with the other CPs surfaces, which can effectively prevent biofouling for more than 14 weeks in the field. The SSCP analysis revealed that the P3 surface may have significant modulating effect on the pioneer microbial communities. The pioneer eukaryotic microbes seemed more susceptible than the pioneer prokaryotic microbes to be subjected to the major perturbations exerted by the P3 surface. The dramatically reduced eukaryotic-microbial diversity may contribute to the impeding and weakening of the development and growth of the biofilm. The P3 surface has the potential to be used for future maritime applications.

Keywords

Anti-biofouling Carboxyl-modified multi-walled carbon nanotubes Polydimethylsiloxane Pioneer microbial communities Single-stranded conformation polymorphism 

Notes

Acknowledgments

The authors express their sincere gratitude and thanks to Shuang Liang and Yongkang Liu of Harbin Institute of Technology, school of Marine science and technology for their constant assistance with the field studies and sampling throughout the course of this investigation. This work was funded by National Natural Science Foundation of China (No. 31071170).

Supplementary material

11274_2016_2094_MOESM1_ESM.tif (3 mb)
Fig. 1 Images of the panels coated with the P0 and P3 surfaces after the immersion in seawater (November 25, 2013 to March 3, 2014 and March, 14 to June 28, 2014, Weihai, China) at the depth of 1.5 m as a function of time (TIFF 3116 kb)
11274_2016_2094_MOESM2_ESM.tif (2.4 mb)
Fig. 2 The SSCP fingerprints of the pioneer microbial communities adhering to the P0 and P3 surfaces. The PP0, PP3 represented the pioneer prokaryotic communities adhering to the P0 and P3 surfaces, whereas the EP0, EP3 represented the pioneer eukaryotes adhering to the P0 and P3 surfaces (TIFF 2456 kb)

References

  1. Antizar-Ladislao B (2008) Environmental levels, toxicity and human exposure to tributyltin (TBT)-contaminated marine environment. A Rev Environ Int 34:292–308. doi: 10.1016/j.envint.2007.09.005 CrossRefGoogle Scholar
  2. Aruoja V, Dubourguier H-C, Kasemets K, Kahru A (2009) Toxicity of nanoparticles of CuO, ZnO and TiO2 to microalgae Pseudokirchneriella subcapitata. Sci Total Environ 407:1461–1468CrossRefGoogle Scholar
  3. Balamurugan P, Joshi MH, Rao TS (2011) Microbial fouling community analysis of the cooling water system of a nuclear test reactor with emphasis on sulphate reducing bacteria. Biofouling 27:967–978. doi: 10.1080/08927014.2011.618636 CrossRefGoogle Scholar
  4. Beigbeder A et al (2008a) Preparation and characterisation of silicone-based coatings filled with carbon nanotubes and natural sepiolite and their application as marine fouling-release coatings. Biofouling 24:291–302. doi: 10.1080/08927010802162885 CrossRefGoogle Scholar
  5. Beigbeder A, Linares M, Devalckenaere M, Degée P, Claes M (2008b) CH–π interactions as the driving force for silicone-based nanocomposites with exceptional properties. Adv Mater 20:1003–1007CrossRefGoogle Scholar
  6. Beigbeder A, Mincheva R, Pettitt ME, Callow ME, Callow JA, Claes M, Dubois P (2010) Marine fouling release silicone/carbon nanotube nanocomposite coatings: on the importance of the nanotube dispersion state. J Nanosci Nanotechnol 10:2972–2978. doi: 10.1166/jnn.2010.2185 CrossRefGoogle Scholar
  7. Beigbeder A et al (2011) Surface and fouling-release properties of silicone/organomodified montmorillonite coatings. J Adhes Sci Technol 25:1689–1700. doi: 10.1163/016942410x524129 CrossRefGoogle Scholar
  8. Briand JF et al (2012) Pioneer marine biofilms on artificial surfaces including antifouling coatings immersed in two contrasting French Mediterranean coast sites. Biofouling 28:453–463. doi: 10.1080/08927014.2012.688957 CrossRefGoogle Scholar
  9. Bulla L (1994) An index of evenness and its associated diversity measure. Oikos 70:167–171CrossRefGoogle Scholar
  10. Camps M et al (2014) Antifouling coatings influence both abundance and community structure of colonizing biofilms: a case study in the Northwestern Mediterranean sea. Appl Environ Microbiol 80:4821–4831CrossRefGoogle Scholar
  11. Caruso T, Pigino G, Bernini F, Bargagli R, Migliorini M (2007) The Berger–Parker index as an effective tool for monitoring the biodiversity of disturbed soils: a case study on Mediterranean oribatid (Acari: Oribatida) assemblages. Biodivers Conserv 16:3277–3285CrossRefGoogle Scholar
  12. Chambers LD, Stokes KR, Walsh FC, Wood RJK (2006) Modern approaches to marine antifouling coatings. Surf Coat Technol 201:3642–3652. doi: 10.1016/j.surfcoat.2006.08.129 CrossRefGoogle Scholar
  13. Ciriminna R, Bright FV, Pagliaro M (2015) Ecofriendly antifouling marine coatings. ACS Sustain Chem Eng 3:559–565. doi: 10.1021/sc500845n CrossRefGoogle Scholar
  14. Efimenko K, Wallace WE, Genzer J (2002) Surface modification of Sylgard-184 poly(dimethyl siloxane) networks by ultraviolet and ultraviolet/ozone treatment. J Colloid Interface Sci 254:306–315CrossRefGoogle Scholar
  15. Eichner CA, Erb RW, Timmis KN, Wagner-Döbler I (1999) Thermal gradient gel electrophoresis analysis of bioprotection from pollutant shocks in the activated sludge microbial community. Appl Environ Microbiol 65:102–109Google Scholar
  16. Fletcher M (1994) Bacterial biofilms and biofouling. Curr Opin Biotechnol 5:302–306CrossRefGoogle Scholar
  17. Gibbs PE (2009) Long-term tributyltin (TBT)-induced sterilization of neogastropods: persistence of effects in Ocenebra erinacea over 20 years in the vicinity of Falmouth (Cornwall, UK). J Mar Biol Assoc UK 89:135–138. doi: 10.1017/s0025315408002336 CrossRefGoogle Scholar
  18. Hinrikson HP, Hurst SF, Lott TJ, Warnock DW, Morrison CJ (2005) Assessment of ribosomal large-subunit D1-D2, internal transcribed spacer 1, and internal transcribed spacer 2 regions as targets for molecular identification of medically important Aspergillus species. J Clin Microbiol 43:2092–2103CrossRefGoogle Scholar
  19. Iijima S, Brabec C, Maiti A, Bernholc J (1996) Structural flexibility of carbon nanotubes. J Chem Phys 104:2089–2092. doi: 10.1063/1.470966 CrossRefGoogle Scholar
  20. Kang S, Pinault M, Pfefferle LD, Elimelech M (2007) Single-walled carbon nanotubes exhibit strong antimicrobial activity. Langmuir 23:8670–8673. doi: 10.1021/la701067r CrossRefGoogle Scholar
  21. Kang S, Herzberg M, Rodrigues DF, Elimelech M (2008) Antibacterial effects of carbon nanotubes: size does matter. Langmuir 24:6409–6413. doi: 10.1021/la800951v CrossRefGoogle Scholar
  22. Lehaitre M, Delauney L, Compère C (2008) Biofouling and underwater measurements real-time observation systems for ecosystem dynamics and harmful algal blooms: theory, instrumentation and modelling. Oceanographic methodology series. UNESCO, Paris, pp 463–493Google Scholar
  23. Ling GC et al (2014) Micro-fabricated polydimethyl siloxane (PDMS) surfaces regulate the development of marine microbial biofilm communities. Biofouling 30:323–335CrossRefGoogle Scholar
  24. Liu Y, Leng C, Chisholm B, Stafslien S, Majumdar P, Chen Z (2013) Surface structures of PDMS incorporated with quaternary ammonium salts designed for antibiofouling and fouling release applications. Langmuir 29:2897–2905. doi: 10.1021/la304571u CrossRefGoogle Scholar
  25. Lu L, Hume ME, Pillai SD (2005) Autoinducer-2-like activity on vegetable produce and its potential involvement in bacterial biofilm formation on tomatoes. Foodborne Pathog Dis 2:242–249CrossRefGoogle Scholar
  26. Miller MB, Bassler BL (2001) Quorum sensing in bacteria. Annu Rev Microbiol 55:165–199CrossRefGoogle Scholar
  27. Peters S, Koschinsky S, Schwieger F, Tebbe CC (2000) Succession of microbial communities during hot composting as detected by PCR-single-strand-conformation polymorphism-based genetic profiles of small-subunit rRNA genes. Appl Environ Microbiol 66:930–936CrossRefGoogle Scholar
  28. Qian P-Y, Lau S, Dahms H-U, Dobretsov S, Harder T (2007) Marine biofilms as mediators of colonization by marine macroorganisms: implications for antifouling and aquaculture Mar. Biotechnol 9:399–410Google Scholar
  29. Sankar GG, Sathya S, Murthy PS, Das A, Pandiyan R, Venugopalan V, Doble M (2015) Polydimethyl siloxane nanocomposites: their antifouling efficacy in vitro and in marine conditions. Int Biodeterior Biodegradation 104:307–314CrossRefGoogle Scholar
  30. Schultz M, Bendick J, Holm E, Hertel W (2011) Economic impact of biofouling on a naval surface ship. Biofouling 27:87–98CrossRefGoogle Scholar
  31. Stach JE, Bathe S, Clapp JP, Burns RG (2001) PCR-SSCP comparison of 16S rDNA sequence diversity in soil DNA obtained using different isolation and purification methods. FEMS Microbiol Ecol 36:139–151CrossRefGoogle Scholar
  32. Stamper DM, Walch M, Jacobs RN (2003) Bacterial population changes in a membrane bioreactor for graywater treatment monitored by denaturing gradient gel electrophoretic analysis of 16S rRNA gene fragments. Appl Environ Microbiol 69:852–860CrossRefGoogle Scholar
  33. Sun Y, Zhang Z (2014) Differential early dynamic process of marine eukaryotic microbial community on anti-biofouling and biofouling-enhancing micro/nano surfaces. J Chem Pharm Res 6(7):183–188Google Scholar
  34. Sun Y, Zhang Z (2016) New anti-biofouling carbon nanotubes-filled polydimethylsiloxane composites against colonization by pioneer eukaryotic microbes. Int Biodeterior Biodegradation 110:147–154. doi: 10.1016/j.ibiod.2016.03.019 CrossRefGoogle Scholar
  35. Sun Y, Lang Y, Sun Q, Liang S, Liu Y, Zhang Z (2016) Effect of anti-biofouling potential of multi-walled carbon nanotubes-filled polydimethylsiloxane composites on pioneer microbial colonization. Colloids Surf B Biointerfaces 145:30–36. doi: 10.1016/j.colsurfb.2016.04.033 CrossRefGoogle Scholar
  36. Thomas G, Clay D, Magurran A (2000) BIODAP-ecological diversity and its measurement. Fundy National Park, Alma, New Brunswick, Canada. http://nhsbig.inhs.uiuc.edu/wes/populations.html. Accessed 2 2006
  37. Tuson HH, Weibel DB (2013) Bacteria-surface interactions. Soft Matter 9:4368–4380. doi: 10.1039/C3sm27705d CrossRefGoogle Scholar
  38. Wang Z, Li J, Zhao J, Xing B (2011) Toxicity and internalization of CuO nanoparticles to prokaryotic alga Microcystis aeruginosa as affected by dissolved organic matter. Environ Sci Technol 45:6032–6040CrossRefGoogle Scholar
  39. Waters CM, Bassler BL (2005) Quorum sensing: cell-to-cell communication in bacteria. Annu Rev Cell Dev Biol 21:319–346CrossRefGoogle Scholar
  40. Zhang Z et al (2011) Surface modification of PDMS by surface-initiated atom transfer radical polymerization of water-soluble dendronized PEG methacrylate. Colloids Surf B Biointerfaces 88:85–92. doi: 10.1016/j.colsurfb.2011.06.019 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.School of Chemistry and Chemical EngineeringHarbin Institute of TechnologyHarbinChina
  2. 2.School of Marine Science and TechnologyHarbin Institute of TechnologyWeihaiChina
  3. 3.Marine Antifouling Engineering Technology Center of Shangdong ProvinceHarbin Institute of TechnologyWeihaiChina

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