Enhanced butanol production from cassava with Clostridium acetobutylicum by genome shuffling
- 485 Downloads
To obtain strains exhibiting high levels of solvent tolerance and butanol production, wild type strains of Clostridium acetobutylicum butanol-producing strain GX01 and Lactobacillus mucosae butanol-tolerant strain M26 were subjected to mutagenesis combining N-methyl-N-nitro-N-nitrosoguanidine induction with genome shuffling. After four successive rounds of genome shuffling, the C. acetobutylicum shuffled strain GS4-3 showing greater levels of fermentation performances (such as secreting a higher level of amylase, improving the thermal stability, and possessing greater environmental robustness) compared to the wild type strains was isolated. As a result, after optimization of culture conditions, mutant GS4-3 produced 32.6 g/L of total solvent, 20.1 g/L of butanol production, and 0.35 g/L/h of butanol productivity, which were, respectively, increased by 23.5, 23.3, and 40.0 % than the wild-type strain GX01, in a 10 L bioreactor. The enhanced production of butanol and tolerance of solvent of mutant associated with GS4-3 make it promising for acetone/butanol/ethanol fermentation from cassava (Manihot esculenta).
KeywordsButanol Cassava Clostridium acetobutylicum N-methyl-N-nitro-N-nitrosoguanidine mutation Genome shuffling
This research was financially supported by the key Project of Guangxi Science and Technology Lab Center (LGZX201006), the National Natural Science Foundation of China (31560027), and the “Bagui Scholars Distinguished Professor” Special Project.
- Li HG, Luo W, Gu QY, Wang Q, Hu WJ, Yu XB (2013) Acetone, butanol, and ethanol production from cane molasses using Clostridium beijerinckii mutant obtained by combined low-energy ion beam implantation and N-methyl-N-nitro-N-nitrosoguanidine induction. Bioresour Technol 137:254–260CrossRefGoogle Scholar
- Minton NP, Morris JG (1983) Regeneration of protoplasts of Clostridium pasteurianum ATCC 6013. J Bacteriol 155:432–434Google Scholar