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A novel enterocin T1 with anti-Pseudomonas activity produced by Enterococcus faecium T1 from Chinese Tibet cheese

  • Hui Liu
  • Lanwei ZhangEmail author
  • Huaxi YiEmail author
  • Xue Han
  • Wei Gao
  • Chunliang Chi
  • Wei Song
  • Haiying Li
  • Chunguang Liu
Original Paper

Abstract

An enterocin-producing Enterococcus faecium T1 was isolated from Chinese Tibet cheese. The enterocin was purified by SP-Sepharose and reversed phase HPLC. It was identified as unique from other reported bacteriocins based on molecular weight (4629 Da) and amino acid compositions; therefore it was subsequently named enterocin T1. Enterocin T1 was stable at 80–100 °C and over a wide pH range, pH 3.0–10.0. Protease sensitivity was observed to trypsin, pepsin, papain, proteinase K, and pronase E. Importantly, enterocin T1 was observed to inhibit the growth of numerous Gram-negative and Gram-positive bacteria including Pseudomonas putida, Pseudomonas aeruginosa, Pseudomonas fluorescens, Escherichia coli, Salmonella typhimurium, Shigella flexneri, Shigella sonnei, Staphylococcus aureus, Listeria monocytogenes. Take together, these results suggest that enterocin T1 is a novel bacteriocin with the potential to be used as a bio-preservative to control Pseudomonas spp. in food.

Keywords

Enterocin Pseudomonas Charaterization Biopreservation 

Notes

Acknowledgments

This work was financially supported by the Research Fund for the Doctoral Program of Higher Education of China (No. 20112302110051), National Science Foundation, China (Nos. 31271906/C2002204, 31571850/C200502).

Compliance with ethical standards

Conflict of interest

No conflict of interest is declared.

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Hui Liu
    • 1
  • Lanwei Zhang
    • 1
    Email author
  • Huaxi Yi
    • 1
    Email author
  • Xue Han
    • 1
  • Wei Gao
    • 1
  • Chunliang Chi
    • 1
  • Wei Song
    • 1
  • Haiying Li
    • 2
  • Chunguang Liu
    • 2
  1. 1.School of Chemical Engineering and TechnologyHarbin Institute of TechnologyHarbinChina
  2. 2.College of Life SciencesHeilongjiang UniversityHarbinChina

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