World Journal of Microbiology and Biotechnology

, Volume 31, Issue 10, pp 1575–1586 | Cite as

The high reutilization value potential of high-salinity anchovy fishmeal wastewater through microbial degradation

  • Juan Gerardo Santoyo Figueroa
  • Hyun Yi Jung
  • Gwi-Taek Jeong
  • Joong Kyun KimEmail author
Original Paper


To provide an option for the reutilization of high-salinity anchovy fishmeal wastewater (FMW), generated during the anchovy fishmeal manufacturing processes, its potential for biodegradation was assessed in 1-l five-neck flasks using a halotolerant and proteolytic microbial consortium. During the first 41 h of biodegradation, the pH, DO, ORP, and dry-sludge weight decreased as the total cell number of the microbial consortium increased steadily; the CODCr/TN ratios remained between 4.0 and 5.5, respectively, indicating the stable metabolic degradation of organic matter. The ORP tended to increase after 41 h, and the unpleasant fishy smell disappeared once positive ORP values were achieved. The removal percentages of CODCr and TN were 59.0 and 54.4 %, respectively, and the dry-sludge weight decreased from 115.5 to 68.0 g, with a degradation rate of 0.59 g h−1, during the 80 h experiment. The supernatant from the culture of the anchovy FMW at 70 h (culture supernatant) was phytotoxin-free, and the level of total amino acids was 8.04 g 100 g−1, comparable to that of commercial fertilizers. In hydroponic cultures containing red bean and barley, the culture supernatant demonstrated a good fertilizing ability. The culture supernatant also exhibited a high degree of antioxidant activity, with a 52.3 % hydroxyl radical-scavenging activity and 0.16 reducing power (at OD 700 nm). Moreover, the culture supernatant inhibited DNA damage from hydroxyl radicals, enhancing the reutilization value of anchovy FMW. This report presents the first description of high-salinity anchovy FMW possessing a high reutilization value potential both for agriculture and medicine.


Reutilization value Anchovy fishmeal wastewater Liquid fertilizer Antioxidant activity DNA-protective activity 



This research was supported by a grant from KOICA (Korea International Cooperation Agency).


  1. Ajibola CF, Fashakin JB, Fagbemi TN, Aluko RE (2011) Effect of peptide size on antioxidant properties of African yam bean seed (Sphenostylis stenocarpa) protein hydrolysate fractions. Int J Mol Sci 12:6685–6702CrossRefGoogle Scholar
  2. Albrecht-Ruiz M, Salas-Maldonado A (2015) Chemical composition of light and dark muscle of Peruvian anchovy (Engraulis ringens) and its seasonal variation. J Aquat Food Prod Technol 24:191–196CrossRefGoogle Scholar
  3. Amir R, Hacham Y, Galili G (2002) Cystathionine γ-synthase and threonine synthase operate in concert to regulate carbon flow towards methionine in plants. Trends Plant Sci 7:153–156CrossRefGoogle Scholar
  4. Azam MS, Kim EJ, Yang HS, Kim JK (2014) High antioxidant and DNA protection activities of N-acetylglucosamine (GlcNAc) and chitobiose produced by exolytic chitinase from Bacillus cereus EW5. SpringerPlus 3:354–364CrossRefGoogle Scholar
  5. Beara IN, Lesjak MM, Jovin ED, Balong KJ, Anačov GT, Orčić DZ, Mimica-Dukić NM (2009) Plantain (Plantago L.) species as novel sources of flavonoid antioxidants. J Agric Food Chem 57:9268–9273CrossRefGoogle Scholar
  6. Bellaaj OG, Jridi M, Khaled HB, Jellouli K, Nasri M (2012) Bioconversion of shrimp shell waste for the production of antioxidant and chitosan used as fruit juice clarifier. Int J Food Sci Technol 47:1835–1841CrossRefGoogle Scholar
  7. Bougatef A (2013) Trypsin from fish processing waste: characteristics and biotechnological applications—comprehensive review. J Clear Prod 57:257–265CrossRefGoogle Scholar
  8. Cira LA, Huerta S, Hall GM, Shirai K (2002) Pilot scale lactic acid fermentation of shrimp wastes for chitin recovery. Process Biochem 37:1359–1366CrossRefGoogle Scholar
  9. Dao VT, Kim JK (2011) Scaled-up bioconversion of fish waste to liquid fertilizer using a 5 l ribbon-type reactor. J Environ Manag 92:2441–2446CrossRefGoogle Scholar
  10. Faid A, Zouiten A, Elmarrakchi A, Achkari-Begdouri A (1997) Biotransformation of fish waste into a stable feed ingredient. Food Chem 60:13–18CrossRefGoogle Scholar
  11. Fang M, Wong JWC (1999) Effects of lime amendment on availability of heavy metals and maturation in sewage sludge composting. Environ Pollut 106:83–89CrossRefGoogle Scholar
  12. Gao MT, Hirata M, Toorisaka E, Hano T (2006) Acid-hydrolysis of fish wastes for lactic acid fermentation. Bioresour Technol 97:2414–2420CrossRefGoogle Scholar
  13. Gao Y, Zhao J, Zu Y, Fu Y, Liang L, Luo M, Wang W, Efferth T (2012) Antioxidant properties, superoxide dismutase and glutathione reductase activities in HepG2 cells with a fungal endophyte producing apigenin from pigeon pea [Cajanus cajan (L.) Millsp.]. Food Res Int 49:147–152CrossRefGoogle Scholar
  14. Gwon BG, Kim JK (2012) Feasibility study on production of liquid fertilizer in a 1 m3 reactor using fishmeal wastewater for commercialization. Environ Eng Res 17:3–8CrossRefGoogle Scholar
  15. Hall GM (1992) Fish processing technology. In: Ockerman HW (ed) Fishery by-products. VCH Publishers, New York, pp 155–192Google Scholar
  16. Hardy RW, Masumoto T (1990) Specifications for marine by-products for aquaculture. In: Keller S (ed) International by-products conference. Alaska Sea Grant Program, Fairbanks, pp 109–120Google Scholar
  17. Hassan TE, Heath JL (1986) Biological fermentation of fish waste for potential use in animal and poultry feeds. Agric Wastes 15:1–15CrossRefGoogle Scholar
  18. Hoekstra NJ, Bosker T, Lantinga EA (2002) Effects of cattle dung from farms with different feeding strategies on germination and initial root growth of cress (Lepidium sativum L.). Agric Ecosyst Environ 93:189–196CrossRefGoogle Scholar
  19. Humphrey A (1998) Shake flask to fermenter: what have we learned? Biotechnol Prog 14:3–7CrossRefGoogle Scholar
  20. Kim JK, Lee G (2009) Aerobically biodegraded fish-meal wastewater as a fertilizer. Environ Res J 3:219–236Google Scholar
  21. Kim JK, Kim JB, Cho KS, Hong YK (2007) Isolation and identification of microorganisms and their aerobic biodegradation of fish-meal wastewater for liquid-fertilization. Int Biodeterior Biodegrad 59:156–165CrossRefGoogle Scholar
  22. Kim JK, Dao VT, Kong IS, Lee HH (2010) Identification and characterization of isolated microorganisms from the viscera of earthworm for reutilization of fish wastes as a liquid-fertilizer. Bioresour Technol 101:5131–5136CrossRefGoogle Scholar
  23. Kim EY, Kim YR, Nam TJ, Kong IS (2012) Antioxidant and DNA protection activities of a glycoprotein isolated from a seaweed, Saccharina japonica. Int J Food Sci Technol 47:1020–1027CrossRefGoogle Scholar
  24. Kim JK, Kim EJ, Kang KH (2014) Achievement of zero emissions by the bioconversion of fishery wastes into fertilizer. In: Lopez-Valdez F, Fernandez-Luqueno F (eds) Fertilizers: components, uses in agriculture and environmental impacts. Nova publishers, New York, pp 69–94Google Scholar
  25. Liao PH, Jones L, Lau AK, Walkemeyer S, Egan B, Holbek N (1997) Composting of fish wastes in a full-scale in-vessel system. Bioresour Technol 59:163–168CrossRefGoogle Scholar
  26. Mathews JA (2012) Green growth strategies—Korean initiatives. Futures 44:761–769CrossRefGoogle Scholar
  27. Parida AK, Das AB (2004) Effect of NaCl stress on nitrogen and phosphorus metabolism in a true mangrove Bruguiera parviflora grown under hydroponic culture. J Plant Physiol 161:921–928CrossRefGoogle Scholar
  28. Park SY, Lee JS, Baek HH, Lee HG (2010) Purification and characterization of antioxidant peptides from soy protein hydrolysate. J Food Biochem 34:120–132CrossRefGoogle Scholar
  29. Ramakrishna H, Sushma SM, Divya R, Mamatharani DR, Panduranga MG (2012) Hydroxy radical and DPPH scavenging activity of crude protein extract of Leucas linifolia: a folk medicinal plant. Asian J Plant Sci Res 2:30–35Google Scholar
  30. Reeve JR, Smith JL, Carpenter-Boggs LC, Reganold JP (2008) Soil-based cycling and differential uptake of amino acids by three species of strawberry (Fragaria spp.) plants. Soil Biol Biochem 40:2547–2552CrossRefGoogle Scholar
  31. Ruiz G, Jeison D, Chamy R (2006) Development of denitrifying and methanogenic activities in USB reactors for the treatment of wastewater: effect of COD/N ratio. Process Biochem 41:1338–1342CrossRefGoogle Scholar
  32. Sachindra NM, Bhaskar N (2008) In vitro antioxidant activity of liquor from fermented shrimp biowaste. Bioresour Technol 99:9013–9016CrossRefGoogle Scholar
  33. Sachindra NM, Bhaskar N, Siddegowda GS, Sathisha AD, Suresh PV (2007) Recovery of cartenoids from ensilaged shrimp waste. Bioresour Technol 98:1642–1646CrossRefGoogle Scholar
  34. Saenjum C, Chaiyavat C, Kadchumsang S, Chansakaow S, Suttajit M (2010) Antioxidant activity and protective effects on DNA damage of Caesalpinia sappan L. extract. J Med Plant Res 4:1594–1600Google Scholar
  35. Vazquez JA, Docasal SF, Prieto MA, Gonzalez MP, Murado MA (2008) Growth and metabolic features of lactic acid bacteria in media with hydrolysed fish viscera. An approach to bio-silage of fishing by-products. Bioresour Technol 99:6246–6257CrossRefGoogle Scholar
  36. Wong JWC, Mak KF, Chan NW, Lam A, Fang M, Zhou LX, Wu QT, Liao XD (2001) Co-composting of soy-bean residues and leaves in Hong Kong. Bioresour Technol 76:99–106CrossRefGoogle Scholar
  37. Wu N, Zu YG, Fu YJ, Kong Y, Zhao JT, Li XJ, Li J, Wink M, Efferth T (2010) Antioxidant activities and xanthine oxidase inhibitory effects of extracts and main polyphenolic compounds obtained from Geranium sibiricum L. J Agric Food Chem 58:4737–4743CrossRefGoogle Scholar
  38. Xu W, Yu G, Xue C, Xue Y, Ren Y (2008) Biochemical changes associated with fast fermentation of squid processing by-products for low salt fish sauce. Food Chem 107:1597–1604CrossRefGoogle Scholar
  39. Yamamoto M, Saleh F, Hayashi F (2004) A fermentation method to dry and convert Shochu distillery by-product to a source of protein and enzymes. J Poult Sci 41:275–280CrossRefGoogle Scholar
  40. Yamamoto M, Saleh F, Ohtsuka A, Hayashi F (2005) New fermentation technique to process fish waste. Anim Sci J 76:245–248CrossRefGoogle Scholar
  41. Yano Y, Oikawa H, Satomi M (2008) Reduction of lipids in fish meal prepared from fish waste by a yeast Yarrowia lipolytica. Int J Food Microbiol 121:302–307CrossRefGoogle Scholar
  42. Zucconi F, Monaco A, Forte M, Beritodi M (1985) Phytotoxins during the stabilization of organic matter. In: Gasser JKR (ed) Composting of agricultural and other wastes. Elsevier, London, pp 73–86Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Juan Gerardo Santoyo Figueroa
    • 1
  • Hyun Yi Jung
    • 1
  • Gwi-Taek Jeong
    • 1
  • Joong Kyun Kim
    • 1
    Email author
  1. 1.Department of Biotechnology and BioengineeringPukyong National UniversityBusanRepublic of Korea

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