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World Journal of Microbiology and Biotechnology

, Volume 31, Issue 10, pp 1517–1527 | Cite as

Bacillus amyloliquefaciens subsp. plantarum GR53, a potent biocontrol agent resists Rhizoctonia disease on Chinese cabbage through hormonal and antioxidants regulation

  • Sang-Mo Kang
  • Ramalingam Radhakrishnan
  • In-Jung LeeEmail author
Original Paper

Abstract

The fungus Rhizoctonia solani is one of the causal agents of numerous diseases that affect crop growth and yield. The aim of this present investigation was to identify a biocontrol agent that acts against R. solani and to determine the agent’s protective effect through phytohormones and antioxidant regulation in experimentally infected Chinese cabbage plants. Four rhizospheric soil bacterial isolates GR53, GR169, GR786, and GR320 were tested for their antagonistic activity against R. solani. Among these isolates, GR53 significantly suppressed fungal growth. GR53 was identified as Bacillus amyloliquefaciens subsp. plantarum by phylogenetic analysis of the 16S rDNA sequence. The biocontrol activity of B. amyloliquefaciens subsp. plantarum GR53 was tested in Chinese cabbage plants under controlled conditions. Results showed that R. solani inhibited plant growth (length, width, fresh and dry weight of leaves) by reducing chlorophyll and total phenolic content, as well as by increasing the levels of salicylic acid, jasmonic acid, abscisic acid, and DPPH scavenging activity. By regulating the levels of these compounds, the co-inoculation of B. amyloliquefaciens subsp. plantarum GR53 heightened induced systemic resistance in infected Chinese cabbage, effectively mitigating R. solani-induced damaging effects and improving plant growth. The results obtained from this study suggest that B. amyloliquefaciens subsp. plantarum GR53 is an effective biocontrol agent to prevent the damage caused by R. solani in Chinese cabbage plants.

Keywords

Bacillus amyloliquefaciens subsp. plantarum GR53 Biocontrol Chinese cabbage Rhizoctonia solani 

Notes

Acknowledgments

This work was financially supported by National Research Foundation of Korea (NRF), Ministry of Science, ICT and Future Planning through Basic Science Research Program (2014R1A1A1004918).

Supplementary material

11274_2015_1896_MOESM1_ESM.docx (510 kb)
Supplementary material 1 (DOCX 509 kb)
11274_2015_1896_MOESM2_ESM.doc (31 kb)
Supplementary material 2 (DOC 31 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Sang-Mo Kang
    • 1
  • Ramalingam Radhakrishnan
    • 1
  • In-Jung Lee
    • 1
    Email author
  1. 1.School of Applied BiosciencesKyungpook National UniversityDaeguRepublic of Korea

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