Green synthesis of copper nanoparticles by Citrus medica Linn. (Idilimbu) juice and its antimicrobial activity

  • Sudhir Shende
  • Avinash P. Ingle
  • Aniket Gade
  • Mahendra Rai
Original Paper


We report an eco-friendly method for the synthesis of copper nanoparticles (CuNPs) using Citron juice (Citrus medica Linn.), which is nontoxic and cheap. The biogenic copper nanoparticles were characterized by UV–Vis spectrophotometer showing a typical resonance (SPR) at about 631 nm which is specific for CuNPs. Nanoparticles tracking analysis by NanoSight-LM20 showed the particles in the range of 10–60 nm with the concentration of 2.18 × 108 particles per ml. X-ray diffraction revealed the FCC nature of nanoparticles with an average size of 20 nm. The antimicrobial activity of CuNPs was determined by Kirby-Bauer disk diffusion method against some selected species of bacteria and plant pathogenic fungi. It was reported that the synthesized CuNPs demonstrated a significant inhibitory activity against Escherichia coli followed by Klebsiella pneumoniae, Pseudomonas aeruginosa, Propionibacterium acnes and Salmonella typhi. Among the plant pathogenic fungi tested, Fusarium culmorum was found to be most sensitive followed by F. oxysporum and F. graminearum. The novelty of this work is that for the first time citron juice was used for the synthesis of CuNPs.


Citron juice (Citrus medica Linn.) Antimicrobial agent Copper nanoparticles Non-toxic Eco-friendly Economical 



The authors are thankful to University Grants commission, New Delhi for financial support under UGC-SAP and API is highly thankful to Department of Science and Technology, New Delhi for providing financial assistance under DST Fast Track Scheme for Young Scientists.


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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Sudhir Shende
    • 1
  • Avinash P. Ingle
    • 1
  • Aniket Gade
    • 1
    • 2
  • Mahendra Rai
    • 1
  1. 1.Department of BiotechnologySant Gadge Baba Amravati UniversityAmravatiIndia
  2. 2.Department of BiologyUtah State UniversityLoganUSA

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