Antimicrobial activity of prodigiosin isolated from Serratia marcescens UFPEDA 398
- 1.2k Downloads
Prodigiosin is an alkaloid and natural red pigment produced by Serratia marcescens. Prodigiosin has antimicrobial, antimalarial and antitumor properties and induces apoptosis in T and B lymphocytes. These properties have piqued the interest of researchers in the fields of medicine, pharmaceutics and different industries. The aim of the present study was to evaluate the antimicrobial activity of prodigiosin against pathogenic micro-organisms. The red pigments produced by S. marcescens exhibited absorption at 534 nm, Rf of 0.59 and molecular weight of 323 m/z. Antimicrobial activity was tested against oxacillin-resistant Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, Streptococcus pyogenes, Acinetobacter sp. and oxacillin-resistant S. aureus. The standard antibiotics employed were ampicillin, chloramphenicol, gentamicin and oxacillin. The disc-diffusion tests demonstrated significant inhibition zones for S. aureus (35 ± 0.6), E. faecalis (22 ± 1.0) and S. pyogenes (14 ± 0.6). However, prodigiosin showed resistance to E. coli, P. aeruginosa and acinetobacter, where no significant formation of inhibitory halos were observed. We determined the inhibitory minimum concentrations and bactericidal for 20 strains of oxacillin-resistant S. aureus (ORSA). The pattern was the antibiotic oxacillin. The minimum inhibitory concentrations observed ranged from 1, 2 and 4.0 μg/mL, respectively, while the minimum bactericidal concentrations ranged from 2, 4, 8 and 16 μg/mL. The S. marcescens prodigiosin produced by showed bactericidal and bacteriostatic effect showing promising antimicrobial activity and suggesting future studies regarding its applicability in antibiotics therapies directed ORSA.
KeywordsAntibiotics Serratia marcescens Resistance Natural products
The authors are grateful to the technical team, the Department of Antibiotics of the Federal University of Pernambuco for offering the use of the laboratories and to the Brazilian fostering agencies CNPq and CAPES.
- Abdullateef AA, Ahmad AAR, Hani AAS, Jalal AB, Mohammad IA, Ghada BS (2012) Documentation of vancomycin-resistant Staphylococcus aureus (VRSA) among children with atopic dermatitis in the Qassim region, Saudi Arabia. Acta Dermatovenerol 21:51–53Google Scholar
- Bauer AW, Kirby WMM, Sherris JC et al (1966) Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496Google Scholar
- Clinical and Laboratory Standards Institute (2010) Document: M100-S20 Vol. 30 No. 1. Replaces M100-S19 Vol. 19:3Google Scholar
- Davaraj N, Dharumaduari D, Noorudin T, Annamalai P (2009) Production of prodigiosin from Serratia marcescens and its cytotoxicity activity. J Pharm Res 2:590–593Google Scholar
- El-Bondkly AM, El-Gendy MM, Bassyouni RH (2012) Overproduction and biological activity of prodigiosin-like pigments from recombinant fusant of endophytic marine Streptomyces species. Antonie Van Leeuwenhoek 102:719–734Google Scholar
- Priya KA et al (2013) Antifouling activity of prodigiosin from estuarine isolate of Serratia marcescens CMST 07. In: Microbiological research in agroecosystem management. Springer, India, pp 11–21Google Scholar
- Vendruscolor F, Fernanda K, de Luana OP, Jorge LN (2007) Production of single cell protein from apple pomace using state solid fermentation. Revista Brasileira de Tecnologi Agroindustrial 01:53–57Google Scholar
- Yang F, Wei HY, Li XQ, Li YH, Li XB, Yin LH, Pu YP (2013) Isolation and characterization of an algicidal bacterium indigenous to Lake Taihu with a red pigment able to lyse Microcystis aeruginosa. Biomed Environ Sci 26(2):148–154Google Scholar