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Analysis of thermophilic fungal populations during phase II of composting for the cultivation of Agaricus subrufescens

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Abstract

The composition and genetic diversity of fungal populations during phase II of compost production for the cultivation of Agaricus subrufescens was determined using culture-dependent and -independent methods on days 3, 6, 10, 12, and 14 of phase II composting. The isolates were morphologically characterized and subsequently analyzed using repetitive extragenic palindromic sequences (rep-PCR), and the intergenic region was sequenced to genetically identify the isolates. Changes on in the filamentous fungi population were analyzed using denaturing gradient gel electrophoresis (DGGE), and the resulting bands were sequenced. The population did not significantly change from day 3 to 10 (2.55 x 105 –6 x 105 CFU g−1), and maximum counts on day 14 of phase II composting (6.92 log CFU g−1). In the morphological characterization, Scytalidium thermophilum, Thermomyces lanuginosus, and Thermomyces ibadanensis were the most abundant identified species. The 26 most abundant isolates identified by morphological analysis were characterized using rep-PCR. A significant amount of genetic diversity was detected among the isolates of all three studied species. Based on the DGGE analysis, the diversity of the fungi was reduced during phase II composting, and S. thermophilum was the predominant species identified throughout the entire process. Thus, this study presents the first report of the involvement of T. ibadanensis in the production of compost for Agaricus mushroom cultivation.

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Acknowledgments

We thank the Foundation for Research Support of Minas Gerais [Fundação de Amparo à Pesquisa de Minas Gerais, FAPEMIG], the Coordination of Improvement of Higher Education Personnel [Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPES] and the National Counsel of Technological and Scientific Development [Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq] for financial support.

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Correspondence to Simone Cristina Marques.

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Souza, T.P., Marques, S.C., da Silveira e Santos, D.M. et al. Analysis of thermophilic fungal populations during phase II of composting for the cultivation of Agaricus subrufescens . World J Microbiol Biotechnol 30, 2419–2425 (2014). https://doi.org/10.1007/s11274-014-1667-3

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Keywords

  • Composting
  • Thermophilic fungi
  • Mushroom
  • Agaricus subrufescens