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Heat-induced oxidative injury contributes to inhibition of Botrytis cinerea spore germination and growth

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Abstract

The inhibitory effect of heat treatment (HT) on Botrytis cinerea, a major postharvest fungal pathogen, and the possible mode of action were investigated. Spore germination and germ tube elongation of B. cinerea were both increasingly and significantly inhibited by HT (43 °C) for 10, 20 or 30 min. HT-induced gene expression of NADPH oxidase A, resulted in the intracellular accumulation of reactive oxygen species. HT-treated B. cinerea spores exhibited higher levels of oxidative damage to proteins and lipids, compared to the non-HT control. These findings indicate that HT resulted in oxidative damage which then played an important role in the inhibitory effect on B. cinerea. In the current study, HT was effective in controlling gray mold, caused by B. cinerea, in pear fruits. Understanding the mode of action by which HT inhibits fungal pathogens will help in the application of HT for management of postharvest fungal diseases of fruits and vegetables.

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Acknowledgments

This work was supported by Key Project from the Government of Anhui Province (No. 2012AKKG0739), Advanced Program of Doctoral Fund of Ministry of Education of China (No. 20110181130009) and National Basic Research Program of China (973 Program) (No. 2011CB100401).

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Correspondence to Jia Liu or Yongsheng Liu.

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Zhao, W., Wisniewski, M., Wang, W. et al. Heat-induced oxidative injury contributes to inhibition of Botrytis cinerea spore germination and growth. World J Microbiol Biotechnol 30, 951–957 (2014). https://doi.org/10.1007/s11274-013-1513-z

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Keywords

  • Fungal pathogen
  • Heat treatment
  • Oxidative stress
  • ROS