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Antimicrobial effect and membrane-active mechanism of tea polyphenols against Serratia marcescens

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Abstract

In this study, we investigated the antimicrobial effect of tea polyphenols (TP) against Serratia marcescens and examined the related mechanism. Morphology changes of S. marcescens were first observed by transmission electron microscopy after treatment with TP, which indicated that the primary inhibition action of TP was to damage the bacterial cell membranes. The permeability of the outer and inner membrane of S. marcescens dramatically increased after TP treatment, which caused severe disruption of cell membrane, followed by the release of small cellular molecules. Furthermore, a proteomics approach based on two-dimensional gel electrophoresis and MALDI-TOF/TOF MS analysis was used to study the difference of membrane protein expression in the control and TP treatment S. marcescens. The results showed that the expression of some metabolism enzymes and chaperones in TP-treated S. marcescens significantly increased compared to the untreated group, which might result in the metabolic disorder of this bacteria. Taken together, our results first demonstrated that TP had a significant growth inhibition effect on S. marcescens through cell membrane damage.

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Acknowledgments

This study were supported by the grant from the The National Natural Science Foundation of China (No. 31301418), (No. 31071514), and (No. 31271954), National Key Technologies R&D Program of China during the 12th Five-Year Plan Period (No. 2012BAD29B06), Most Important Program for doctor culture Program Foundation of Education Department of Central Government (No. 20113326130001), Food Safety Key Laboratory of Liaoning Province and Engineering and Technology Research Center of Food preservation, Processing and Safety Control of Liaoning Province, The Major Programs for the Science and Technology of Zhejiang Province (No. 2009C03017-5), The Program for the Science & Technology of Zhejiang Province (2012C22049).

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Correspondence to Jianrong Li.

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Yi, S., Wang, W., Bai, F. et al. Antimicrobial effect and membrane-active mechanism of tea polyphenols against Serratia marcescens . World J Microbiol Biotechnol 30, 451–460 (2014). https://doi.org/10.1007/s11274-013-1464-4

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Keywords

  • Tea polyphenols
  • Serratia marcescens
  • Membrane-active mechanism