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World Journal of Microbiology and Biotechnology

, Volume 30, Issue 2, pp 539–545 | Cite as

Cyanobacteria as a source of hydrogen for methane formation

  • Andreas BergEmail author
  • Peter Lindblad
  • Bo Håkan Svensson
Original Paper

Abstract

In a study during the 1970s co-variation of nitrogenase activity and methane formation associated with Sphagnum riparium was observed. This was suggested as evidence for a possible mechanism of hydrogen transfer from cyanobacteria to methanogens. We show experimentally that such a pathway is feasible. In a series of laboratory experiments, using a hydrogenase deficient strain of the heterocystous cyanobacterium Nostoc punctiforme and the hydrogenotrophic methanogen Methanospirillum hungateii in co-cultures, increasing light intensities resulted in elevated nitrogenase activity and methane production. The increase in methane production can be directly deduced from the nitrogenase activity of the N. punctiforme based on hydrogen balance calculations. These experimental results clearly suggest the possible existence of a novel photosynthetically regulated pathway for methane formation.

Keywords

Cyanobacteria Hydrogen production Methane production Methanogenic archaea 

Notes

Acknowledgments

This work was funded by the Swedish Research Council and the Swedish Energy Agency. The authors are grateful to Dr Pia Lindberg at Uppsala University for supplying the N. punctiforme strain NMH5.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Andreas Berg
    • 1
    Email author
  • Peter Lindblad
    • 2
  • Bo Håkan Svensson
    • 1
  1. 1.Department of Water and Environmental StudiesUniversity of LinköpingLinköpingSweden
  2. 2.Microbial Chemistry, Department of Chemistry, Ångström LaboratoryUppsala UniversityUppsalaSweden

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