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World Journal of Microbiology and Biotechnology

, Volume 30, Issue 2, pp 429–437 | Cite as

Biodegradation of malachite green by Ochrobactrum sp.

  • S. R. Vijayalakshmidevi
  • Karuppan MuthukumarEmail author
Original Paper

Abstract

This study presents the biodegradation of malachite green (MG), a triphenylmethane dye, using a novel microorganism isolated from textile effluent contaminated environment. The organism responsible for degradation was identified as Ochrobactrum sp JN214485 by 16S rRNA analysis. The effect of operating parameters such as temperature, pH, immobilized bead loading, and initial dye concentration on % degradation was studied, and their optimal values were found to be 30 °C, 6, 20 g/L and 100 mg/L, respectively. The analysis showed that the extracellular enzymes were responsible for the degradation. The biodegradation of MG was confirmed by UV–visible spectroscopic and FTIR analysis. The phytotoxicity test concluded that the degradation products were less toxic compared to MG. The kinetics of biodegradation was studied and the activation energy was found to be 10.65 kcal/mol.

Keywords

Malachite green Ochrobactrum sp. Biodegradation Textile effluent isolate Kinetics 

Notes

Acknowledgments

This work was funded by the Department of Science & Technology (DST), New Delhi under WOS-A project. Ms. S.R. Vijayalakshmidevi expresses her sincere thanks to the DST.

References

  1. Ayed L, Chaieb K, Cheref A, Bakhrouf A (2009) Biodegradation of triphenylmethane dye Malachite Green by Sphingomonas paucimobilis. World J Microbiol Biotechnol 25:705–711CrossRefGoogle Scholar
  2. Ayed L, Achour S, Bakhrouf A (2011) Application of the mixture design to decolorize effluent textile wastewater using continuous stirred bed reactor, Water SA,37. ISSN: 0378-4738Google Scholar
  3. Banet IM, Nigam P, Singh D, Marchant R (1996) Microbial decolorization of textile dye containing effluent a review. Bioresour Technol 58:217–227CrossRefGoogle Scholar
  4. Benefield LD, Randall CW (1980) Biological process design for waste water treatment. Prentice-Hall, Inc., Englewood Cliffs, pp 1–15Google Scholar
  5. Berberidou C, Poulios I, Xekoukoulotakis NP, Mantzavinos D (2007) Sonolytic, photocatalytic and sonophotocatalytic degradation of malachite green in aqueous solutions. Appl Catal B 74:63–72CrossRefGoogle Scholar
  6. Chang C, Tseng SK (1998) Immobilisation of Alcaligenes eutrophus using PVA cross linked with sodium nitrate. Biotechnol Tech 12:865–868CrossRefGoogle Scholar
  7. Chang JC, Doerge DR, Cerniglia CE (2001) Biotransformation of malachite green by the fungus Cunninghamella elegans. Appl Environ Microbiol 67(9):4358–4360CrossRefGoogle Scholar
  8. Chen BY (2002) Understanding decolurization characteristics of reactive azo dyes by Pseudomonas lutela: toxicity and kinetics. Process Biochem 38:437–446CrossRefGoogle Scholar
  9. Chen CY, Kuob JT, Cheng CY, Huang YT, Hob IH, Chung YC (2009) Biological decolorization of dye solution containing malachite green by Pandoraea pulmonicola YC32 using a batch and continuous system. J Hazard Mater 172:1439–1445CrossRefGoogle Scholar
  10. Dafale N, Nageswara Rao N, Mesham SU, Wate SR (2007) Decolorization of azo dyes and simulated dye bath waste water using acclimatized microbial consortium-Biostimulation and halo tolerance. Bioresour Technol 99:2552–2558CrossRefGoogle Scholar
  11. Daneshvar N, Ayazloo M, Khataee AR, Pourhassan M (2006) Biological decolourization of dye solution containing Malachite Green by microalgae Cosmarium sp. Bioresour Technol 98:1176–1182CrossRefGoogle Scholar
  12. El-Naas MH, Al-Muhtasheb SA, Makhlouf S (2009) Biodegradation of phenol by Pseudomoans putida in polyvinyl alcohol(PVA) gel. J Hazard Mater 164:720–725CrossRefGoogle Scholar
  13. Forgacs E, Cserhati T, Oros G (2004) Removal of synthetic dyes from wastewaters: a review. Environ Int 30:953CrossRefGoogle Scholar
  14. Hameed BH, Lee TW (2009) Degradation of malachite green in aqueous solution by Fenton process. J Hazard Mater 164:468–472CrossRefGoogle Scholar
  15. Ivanova E, Chipeva V, Ivanova I, Dousset X, Poncelet D (2002) Encapsulation of lactic acid bacteria in calcium alginate beads for bacteriocin production. J Cult Collect 32:53–58Google Scholar
  16. Jadhav JP, Govindwar SP (2006) Biotransformation of malachite green by Saccharomyces cerevisiae MTCC 463. Yeast 23:315–323CrossRefGoogle Scholar
  17. Jadhav SB, Phugare SS, Patil PS, Jadhav JP (2011) Biochemical degradation pathway of textile dye Remazol red and subsequent toxicological evaluation, cytotoxicity, genotoxicity and oxidative stress studies. International Biodeteroriation and Biodegradation 1–11Google Scholar
  18. Jefferson J, Joseph OF (2003) Decolorization of malachite green and crystal violet by waterborne pathogenic mycobacteria. Antimicrob Agents Chemother 47(7):2323–2326CrossRefGoogle Scholar
  19. Johnsen A, Flink JM (1986) Influence of alginate properties and gel reinforcement on fermentation characteristics of immobilized yeast cells. Enzy Microb Technol 8:737–748CrossRefGoogle Scholar
  20. Juan Y, Jun D, Zhao JC, Miao J (2010) Mechanism of photocatalytic degradation of dye MG by TiO2-film electrode with cathodic bias potential. J Phys Chem 55(2):131–139Google Scholar
  21. Khataee AR, Zarei M, Dehghan G, Ebadi E, Pourhassan M (2011) Biotreatment of a triphenylmethane dye solution using a Xanthophyta alga: modeling of key factors by neural network. J Taiwan Inst Chem Eng 42:380–386CrossRefGoogle Scholar
  22. Mahmood Q, Hu B, Cai J, Zheng P, Azim MR, Jilani G, Islama E (2009) Isolation of Ochrobactrum sp.QZ2 from sulfide and nitrite treatment system. J Hazard Mater 165:558–565CrossRefGoogle Scholar
  23. Masalma MA, Armougom F, Scheld WM, Dufour H, Roche PH, Drancourt M, Raoult D (2009) The expansion of the microbiological spectrum of brain abscesses with use of multiple 16S Ribosomal DNA sequencing. Clin Infect Dis 48:1169–1178CrossRefGoogle Scholar
  24. Mittal A (2006) Adsorption kinetics of removal of a toxic dye, Malachite Green, from wastewater by using hen feathers. J Hazard Mater 133:196–202CrossRefGoogle Scholar
  25. Pandey A, Nigam P, Soccol CR, Soccol VT, Singh D, Mohan R (2000) Advances in microbial amylases. Biotechnol Appl Biochem 31:135–152CrossRefGoogle Scholar
  26. Papinutti L, Mouso N, Forchiassin F (2006) Removal and degradation of the fungicide dye malachite green from aqueous solution using the system wheat bran–Fomes sclerodermeus. Enzyme Microbial Technol 39:848–853CrossRefGoogle Scholar
  27. Parshetti G, Kalme S, Saratale G, Govindwar S (2006) Biodegradation of Malachite Green by Kocuria rosea MTCC 15. Acta Chim Slov 53:493–498Google Scholar
  28. Qiu X, Zhong Q, Lia M, Baia W, Lic B (2007) Biodegradation of p-nitrophenol by methyl parathion-degrading Ochrobactrum sp. B2. Int Biodeterior Biodegradation 59:297–301CrossRefGoogle Scholar
  29. Rai H, Bhattacharya M, Singh J, Bansal TK, Vats P, Banerjee UC (2005) Removal of Dyes from the effluent of textile and dyestuff manufacturing industry: a review of emerging techniques with reference to biological treatment. Crit Rev Environ Sci Technol 35:219CrossRefGoogle Scholar
  30. Satish CM, James TS (2000) Immobilization of chymotrypsin for use in batch and continuous reactors. J Chem Technol Biotechnol 75:519–525CrossRefGoogle Scholar
  31. Srivastava S, Sinha R, Roy D (2004) Toxicological effects of Malachite Green. Aqua Toxicol 66:319–329CrossRefGoogle Scholar
  32. Telke A, Kalyani D, Jadhav J, Govindwar S (2008) Kinetics and Mechanism of Reactive Red 141 Degradation by a Bacterial Isolate Rhizobium radiobacter MTCC 8161. Acta Chim Slov 55:320–329Google Scholar
  33. Zhang F, Yediler A, Liang X, Kettrup A (2004) Effects of dye additives on the ozonation process and oxidation by-products: a comparative study using hydrolyzed CI Reactive Red 120. Dyes Pigments 60(1):1–7Google Scholar
  34. Zhang L, Su M, Liu N, Zhou X, Kang P (2009) Degradation of malachite green solution using combined microwave and ZnFe(2)O(4) powder. Water Sci Technol 60(10):2563–2569CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Department of Chemical Engineering, Alagappa College of TechnologyAnna UniversityChennaiIndia

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