World Journal of Microbiology and Biotechnology

, Volume 30, Issue 2, pp 429–437 | Cite as

Biodegradation of malachite green by Ochrobactrum sp.

  • S. R. Vijayalakshmidevi
  • Karuppan MuthukumarEmail author
Original Paper


This study presents the biodegradation of malachite green (MG), a triphenylmethane dye, using a novel microorganism isolated from textile effluent contaminated environment. The organism responsible for degradation was identified as Ochrobactrum sp JN214485 by 16S rRNA analysis. The effect of operating parameters such as temperature, pH, immobilized bead loading, and initial dye concentration on % degradation was studied, and their optimal values were found to be 30 °C, 6, 20 g/L and 100 mg/L, respectively. The analysis showed that the extracellular enzymes were responsible for the degradation. The biodegradation of MG was confirmed by UV–visible spectroscopic and FTIR analysis. The phytotoxicity test concluded that the degradation products were less toxic compared to MG. The kinetics of biodegradation was studied and the activation energy was found to be 10.65 kcal/mol.


Malachite green Ochrobactrum sp. Biodegradation Textile effluent isolate Kinetics 



This work was funded by the Department of Science & Technology (DST), New Delhi under WOS-A project. Ms. S.R. Vijayalakshmidevi expresses her sincere thanks to the DST.


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Department of Chemical Engineering, Alagappa College of TechnologyAnna UniversityChennaiIndia

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