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World Journal of Microbiology and Biotechnology

, Volume 30, Issue 1, pp 125–133 | Cite as

Temperature influences β-carotene production in recombinant Saccharomyces cerevisiae expressing carotenogenic genes from Phaffia rhodozyma

  • Feng ShiEmail author
  • Wubing Zhan
  • Yongfu Li
  • Xiaoyuan Wang
Original Paper

Abstract

Red yeast Phaffia rhodozyma is a prominent microorganism able to synthesize carotenoid. Here, three carotenogenic cDNAs of P. rhodozyma CGMCC 2.1557, crtE, crtYB and crtI, were cloned and introduced into Saccharomyces cerevisiae INVSc1. The recombinant Sc-EYBI cells could synthesize 258.8 ± 43.8 μg g−1 dry cell weight (DCW) of β-carotene when growing at 20 °C, about 59-fold higher than in those growing at 30 °C. Additional expression of the catalytic domain of 3-hydroxy-3-methylglutaryl-coenzyme A reductase from S. cerevisiae (Sc-EYBIH) increased the β-carotene level to 528.8 ± 13.3 μg g−1 DCW as cells growing at 20 °C, 27-fold higher than cells growing at 30 °C, although cells grew faster at 30 °C than at 20 °C. Consistent with the much higher β-carotene level in cells growing at 20 °C, transcription level of three crt genes and cHMG1 gene in cells growing at 20 °C was a little higher than in those growing at 30 °C. Meanwhile, expression of three carotenogenic genes and accumulation of β-carotene promoted cell growth. These results reveal the influence of temperature on β-carotene biosynthesis and may be helpful for improving β-carotene production in recombinant S. cerevisiae.

Keywords

β-Carotene Carotenogenic genes cHMG1 Phaffia rhodozyma Saccharomyces cerevisiae 

Notes

Acknowledgments

This work was supported by the grants from the National Natural Science Foundation of China (No. 30870056).

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Feng Shi
    • 1
    • 2
    Email author
  • Wubing Zhan
    • 1
    • 2
  • Yongfu Li
    • 3
  • Xiaoyuan Wang
    • 1
    • 2
  1. 1.State Key Laboratory of Food Science and TechnologyJiangnan UniversityWuxiChina
  2. 2.The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of BiotechnologyJiangnan UniversityWuxiChina
  3. 3.National Engineering Laboratory for Cereal Fermentation TechnologyJiangnan UniversityWuxiChina

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