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World Journal of Microbiology and Biotechnology

, Volume 29, Issue 12, pp 2373–2378 | Cite as

Sodium houttuyfonate, a potential phytoanticipin derivative of antibacterial agent, inhibits bacterial attachment and pyocyanine secretion of Pseudomonas aeruginosa by attenuating flagella-mediated swimming motility

  • Jing Shao
  • Huijuan ChengEmail author
  • Changzhong Wang
  • Daqiang Wu
  • Xiaoli Zhu
  • Lingling Zhu
  • Zhenxin Sun
Original Paper

Abstract

Pseudomonas aeruginosa is a well-known clinical pathogen for its recalcitrant infection caused by biofilm formation which are initiated by flagella-mediated attachment. Sodium houttuyfonate (SH) is a natural phytoanticipin derivative of houttuynin and has anti-pathogenic effect on P. aeruginosa biofilm formation. In this paper, when using 1/2 × MIC SH, the diameter of P. aeruginosa swimming motility was sharply shortened to 36 % in 24 h incubation, and the fold changes of fliC required for swimming motility was 0.36 in 24 h cultivation, the adherence inhibition accounted for about 46 %, and the pyocyanin production decreased to 47 % after 1-day treatment and 56 % after 3-day treatment with obvious visual changes from dark green to light green, compared with the negative control. With the help of mass spectra and scanning electronic microscope, 1/2 × MIC SH was further testified to be enough to eradicate flagella and inhibit pyocyanin secretion of P. aeruginosa. The results do not only re-affirm the close interplay of attachment and virulence (i.e. swimming motility and pyocyanin), but also unravel the potential mechanism of SH on anti-biofilm of P. aeruginosa.

Keywords

Pseudomonas aeruginosa Attachment Sodium houttuyfonate Swimming motility Pyocyanin 

Abbreviations

SH

Sodium houttuyfonate

MS

Mass spectrum

SEM

Scanning electronic microscope

AZM

Azithromycin

MIC

Minimal inhitory concentration

PBS

Phosphate buffer saline

SD

Standard deviation

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 81173629).

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

11274_2013_1405_MOESM1_ESM.doc (62 kb)
Supplementary material 1 (DOC 61 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Jing Shao
    • 1
  • Huijuan Cheng
    • 1
    Email author
  • Changzhong Wang
    • 1
  • Daqiang Wu
    • 1
  • Xiaoli Zhu
    • 1
  • Lingling Zhu
    • 1
  • Zhenxin Sun
    • 1
  1. 1.Laboratory of Microbiology and Immunology, School of Chinese and Western Integrative MedicineAnhui University of Chinese MedicineHefeiChina

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