Identification of a potential fungal species by 18S rDNA for ligninases production
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Fungal species for ligninases production was investigated by 18S ribosomal DNA sequence analysis. Two primer sets were chosen to amplify a major part of the 18S rDNA, which resulted in intense PCR product of approximately 550–820 bp in size per sample. The results suggest that the 18S rDNA-based approach is a useful tool for identification of unknown potential fungal species for ligninases production. The isolated fungal species produces mainly manganese peroxidase (MnP). The enzyme oxidized a variety of the usual MnP substrates, including lignin related polyphenols. Time course studies showed that maximum production of ligninolytic enzymes MnP (64 IU L−1), lignin peroxidase (26.35 IU L−1), and laccase (5.44 IU L−1), respectively, were achieved after 10 days of cultivation under optimum conditions. Furthermore, the biological decolorization of Remazol Brilliant Blue R dye following 10 days of cultivation was 94 %. NCBI BLAST was used to search for closest matched sequences in the GenBank database and based on sequence homology the first BLAST hit was Dothioraceae sp. LM572 with accession number EF060858.1.
KeywordsDothideomycetes 18S rDNA PCR Ligninases Ascomycetes
This work was funded by CAPES (Brazil) and DFAIT (Canada).
- Cannon PF, Kirk PM (2007) Fungal families of the World. Wallingford: CABI. pp. 109–110. ISBN 0-85199-827-5Google Scholar
- Mahdi LE, Donachie SP (2008) Dothioraceae sp. LM572 18S ribosomal RNA gene, partial sequence. EF060858.1, GI: 117653263, 791 bp DNA linear ENV 11-DEC-2008Google Scholar
- White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic, New York, pp 315–322Google Scholar