World Journal of Microbiology and Biotechnology

, Volume 29, Issue 11, pp 2095–2103 | Cite as

Characterization and constitutive expression of an acidic mesophilic endo-1,4-β-d-xylanohydrolase with high thermotolerance and catalytic efficiency in Pichia pastoris

  • Ning Guo
  • Jia Zheng
  • Jian Tian
  • Lishuang Wu
  • Hongbo Zhou
Original Paper


A putative endo-1,4-β-d-xylanohydrolase gene xyl11 from Aspergillus niger, encoding a 188-residue xylanase of glycosyl hydrolase family 11, was constitutively expressed in Pichia pastoris. The recombinant Xyl11 exhibited optimal activity at pH 5.0 and 50 °C, and displayed more than 68 % of the maximum activity over the temperature range 35–65 °C and 33 % over the pH range 2.2–7.0. It maintained more than 40 % of the original activity after incubation at 90 °C (pH 5.0) for 10 min and more than 75 % of the original activity after incubation at pH 2.2–11.0 (room temperature) for 2 h. The specific activity, K m and V max of purified Xyl11 were 22,253 U mg−1, 6.57 mg ml−1 and 51,546.4 μmol min−1 mg−1. It could degrade xylan to a series of xylooligosaccharides and no xylose was detected. The recombinant enzyme with high stability and catalytic efficiency could work over wide ranges of pH and temperature and thus has the potential for various industrial applications.


Endo-1,4-β-d-xylanohydrolase De novo synthesis Aspergillusniger Pichia pastoris Constitutive expression 



This work was financially supported by Genencor Innovation Grant.

Supplementary material

11274_2013_1374_MOESM1_ESM.docx (17 kb)
Supplementary material 1 (DOCX 16 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Ning Guo
    • 1
  • Jia Zheng
    • 1
  • Jian Tian
    • 1
  • Lishuang Wu
    • 1
  • Hongbo Zhou
    • 1
    • 2
  1. 1.School of Minerals Processing and BioengineeringCentral South UniversityChangshaPeople’s Republic of China
  2. 2.Key Laboratory of Biometallurgy, Ministry of EducationCentral South UniversityChangshaPeople’s Republic of China

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