World Journal of Microbiology and Biotechnology

, Volume 29, Issue 10, pp 1773–1781 | Cite as

Andrimid production at low temperature by a psychrotolerant Serratia proteamaculans strain

  • Leandro A. Sánchez
  • Manuel González Sierra
  • Faustino Siñeriz
  • Osvaldo DelgadoEmail author
Original Paper


Andrimid, a known non-ribosomal pseudo-peptide antibiotic, was isolated from a psychrotolerant Serratia proteamaculans strain. The antibiotic peptide was produced at low temperature (8 °C) in a 7.5 l BIOFLO 101 bioreactor under batch culture mode. Andrimid activity from S. proteamaculans culture was only detected at 25 °C and below and potent antibacterial activity was revealed against both, pathogenic and non-pathogenic bacteria. Minimal inhibitory concentration values determined by microdilution experiments varied in the range between 0.01 and 0.78 μg/ml. Antimicrobial purification and structure elucidation were carried out by LC-MS/MS and 1H/13C NMR approaches. The effects on the ultrastructure of sensitive Escherichia coli 35,218 cells were observed by transmission electron microscopy at different inhibition stages. This work demonstrated the significance of bioprospection from cold environments through the screening of microorganisms with ability to produce cold-active biomolecules of biotechnological interest. S. proteamaculans 136 was revealed as a novel microbial source for andrimid production at low temperatures, showing biotechnological potential to be applied in cryopreservation, food or cosmetic industries against pathogenic bacteria.


Andrimid Psychrotolerant Serratia proteamaculans Antimicrobial Pseudo-peptide 

Supplementary material

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Supplementary material 1 (TIFF 1143 kb)
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Supplementary material 2 (TIFF 7348 kb)
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Supplementary material 3 (PDF 159 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Leandro A. Sánchez
    • 1
  • Manuel González Sierra
    • 2
  • Faustino Siñeriz
    • 1
  • Osvaldo Delgado
    • 1
    Email author
  1. 1.PROIMI-CONICETSan Miguel de TucumánArgentina
  2. 2.Fac. de Cs Bioq. y Farm., IQUIRUNRRosarioArgentina

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