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World Journal of Microbiology and Biotechnology

, Volume 29, Issue 8, pp 1481–1486 | Cite as

Candida konsanensis sp. nov., a new yeast species isolated from Jasminum adenophyllum in Thailand with potentially carboxymethyl cellulase-producing capability

  • Somporn Sarawan
  • Polson Mahakhan
  • Sasitorn Jindamorakot
  • Kanit Vichitphan
  • Sukanda Vichitphan
  • Jutaporn Sawaengkaew
Original Paper

Abstract

A new yeast species (KKU-FW10) belonging to the Candida genus was isolated from Jasminum adenophyllum in the Plant Genetic Conservation Project under The Royal Initiative of Her Royal Highness Princess Maha Chakri Sirindhorn area, Chulabhorn Dam, Konsan district within Chaiyaphum province in Thailand. The strain was identified via analysis of nucleotide sequences from the D1/D2 domain of 26S ribosomal DNA and based on its morphological, physiological and biochemical characteristics. The sequence obtained from yeast isolate KKU-FW10 was 97 percent identical to that of Candida chanthaburiensis (GenBank accession number AB500861.1), with 506/517 (nucleotides identity/total nucleotides) matching nucleotides, nine substitutions and two gaps being detected. This species belonged to the Candida clade. Regarding morphological characteristics, isolate KKU-FW10 presents cream-colored butyrous colonies, vegetative reproduction through budding and, round cells without filaments or ascospores. The major ubiquinone detected was Q-9. The above results suggest that isolate KKU-FW10 is a new member of the genus Candida, and the name Candida konsanensis is proposed for this yeast. The type strain of the new species is KKU-FW10T (= BCC 52588T, = NBRC 109082T, = CBS 12666T). In addition, this KKU-FW10 could potentially produce 58.24 Units/ml of carboxymethyl cellulase when it was cultured in YP broth containing 1.0 % carboxymethyl cellulose for 24 h.

Keywords

New yeast species D1/D2 domain Candida konsanensis Jasminum adenophyllum Carboxymethyl cellulase 

Notes

Acknowledgments

The authors thank the Plant Genetic Conservation Project under The Royal Initiative of Her Royal Highness Princess Maha Chakri Sirindhorn: Khon Kaen University for permission to conduct the research and the Electricity Generating Authority of Thailand (EGAT) for field support. This work was partially supported by the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission, through the Biofuel Cluster of Khon Kaen University. We thank to KKU Publication Clinic for providing professional editing service from American Journal Experts.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Somporn Sarawan
    • 1
  • Polson Mahakhan
    • 2
  • Sasitorn Jindamorakot
    • 3
  • Kanit Vichitphan
    • 4
    • 5
  • Sukanda Vichitphan
    • 4
    • 5
  • Jutaporn Sawaengkaew
    • 2
  1. 1.Graduate SchoolKhon Kaen UniversityKhon KaenThailand
  2. 2.Department of Microbiology, Faculty of ScienceKhon Kaen UniversityKhon KaenThailand
  3. 3.BIOTEC Culture CollectionNational Center for Genetic Engineering and Biotechnology (BIOTEC)Klong LuangThailand
  4. 4.Department of Biotechnology, Faculty of TechnologyKhon Kaen UniversityKhon KaenThailand
  5. 5.Fermentation Research Center for Value Added Agricultural Products (FerVAAP)Khon Kaen UniversityKhon KaenThailand

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