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World Journal of Microbiology and Biotechnology

, Volume 29, Issue 8, pp 1453–1460 | Cite as

Halotolerant, alkaliphilic urease-producing bacteria from different climate zones and their application for biocementation of sand

  • Viktor Stabnikov
  • Chu Jian
  • Volodymyr Ivanov
  • Yishan Li
Original Paper

Abstract

Microbially induced calcium carbonate precipitation (MICP) is a phenomenon based on urease activity of halotolerant and alkaliphilic microorganisms that can be used for the soil bioclogging and biocementation in geotechnical engineering. However, enrichment cultures produced from indigenous soil bacteria cannot be used for large-scale MICP because their urease activity decreased with the rate about 5 % per one generation. To ensure stability of urease activity in biocement, halotolerant and alkaliphilic strains of urease-producing bacteria for soil biocementation were isolated from either sandy soil or high salinity water in different climate zones. The strain Bacillus sp. VUK5, isolated from soil in Ukraine (continental climate), was phylogenetically close in identity (99 % of 16S rRNA gene sequence) to the strain of Bacillus sp. VS1 isolated from beach sand in Singapore (tropical rainforest climate), as well as to the strains of Bacillus sp. isolated by other researchers in Ghent, Belgium (maritime temperate climate) and Yogyakarta, Indonesia (tropical rainforest climate). Both strains Bacillus sp. VS1 and VUK5 had maximum specific growth rate of 0.09/h and maximum urease activities of 6.2 and 8.8 mM of hydrolysed urea/min, respectively. The halotolerant and alkaliphilic strain of urease-producing bacteria isolated from water of the saline lake Dead Sea in Jordan was presented by Gram-positive cocci close to the species Staphylococcus succinus. However, the strains of this species could be hemolytic and toxigenic, therefore only representatives of alkaliphilic Bacillus sp. were used for the biocementation studies. Unconfined compressive strengths for dry biocemented sand samples after six batch treatments with strains VS1and VUK5 were 765 and 845 kPa, respectively. The content of precipitated calcium and the strength of dry biocemented sand at permeability equals to 1 % of initial value were 12.4 g Ca/kg of dry sand and 454 kPa, respectively, in case of biocementation by the strain VS1. So, halotolerant, alkaliphilic, urease-producing bacteria isolated from different climate zones have similar properties and can be used for biocementation of soil.

Keywords

Biocementation Climate zones Urease-producing bacteria 

Notes

Acknowledgments

This research was supported in part by the grant P0820014 “Biocement—a new sustainable and energy saving material for construction and waste treatment” from the Agency for Science, Technology and Research (A*STAR), Singapore.

References

  1. Al-Thawadi S, Cord-Ruwisch R (2012) Calcium carbonate crystals formation by ureolytic bacteria isolated from Australian soil and sludge. J Adv Sci Eng Res 2:12–26Google Scholar
  2. American Public Health Association (APHA) (1999) Standard methods for the analysis of water and wastewater, 20th edn. American Public Health Association, WashingtonGoogle Scholar
  3. Bachmeier KL, Williams AE, Warmington JR, Bang SS (2002) Urease activity in microbiologically-induced calcite precipitation. J Biotechnol 93:171–181CrossRefGoogle Scholar
  4. Bang SS, Galinat JK, Ramakrishnan V (2001) Calcite precipitation induced by polyurethane-immobilized Bacillus pasteurii. Enzyme Microb Technol 28:404–409CrossRefGoogle Scholar
  5. Burbank MB, Weaver TJ, Green TL, Williams BC, Crawford RL (2011) Precipitation of calcite by indigenous microorganisms to strengthen liquefiable soil. Geomicrobiol J 28:301–312CrossRefGoogle Scholar
  6. Burbank MB, Weaver TJ, Williams BC, Crawford RL (2012) Urease activity of ureolytic bacteria isolated from six soils in which calcite was precipitated by indigenous bacteria. Geomicrobiol J 29:389–395CrossRefGoogle Scholar
  7. Christians S, Jose J, Schäfer U, Kaltwasser H (1991) Purification and subunit determination of the nickel-dependent Staphylococcus xylosus urease. FEMS Microbiol Lett 80:271–275CrossRefGoogle Scholar
  8. Chu J, Ivanov V, He J, Naeimi M, Li B, Stabnikov V (2011) Development of microbial geotechnology in Singapore. In: Han J, Alzamora DA (eds) ASCE GeoFrontiers: Adv Geotech Eng 211:4070–4078Google Scholar
  9. Chu J, Ivanov V, Stabnikov V, He J, Li B, Naemi M (2012a) Biocement: green building- and energy-saving material. Adv Mat Res 347–353:4051–4054Google Scholar
  10. Chu J, Stabnikov V, Ivanov V (2012b) Microbially induced calcium carbonate precipitation on surface or in the bulk of soil. Geomicrobiol J 29:544–549CrossRefGoogle Scholar
  11. De Muynck W, Cox K, Verstraete W, De Belie N (2008a) Bacterial carbonate precipitation as an alternative surface treatment for concrete. Constr Build Mater 22:875–885CrossRefGoogle Scholar
  12. De Muynck WD, Debrouwer D, De Belie ND, Verstraete W (2008b) Bacterial carbonate precipitation improves the durability of cementitious materials. Cem Concr Res 38:1005–1014CrossRefGoogle Scholar
  13. De Muynck W, De Belie N, Verstraete W (2010) Microbial carbonate precipitation in construction materials: a review. Ecol Eng 36:18–136Google Scholar
  14. DeJong J, Fritzges M, Nusstein K (2006) Microbially induced cementation to control sand response to undrained shear. J Geotech Geoenviron Eng 132:1381–1392CrossRefGoogle Scholar
  15. DeJong JT, Mortensen BM, Martinez BC, Nelson DC (2010) Bio-mediated soil improvement. Ecol Eng 36:197–210CrossRefGoogle Scholar
  16. Fernandes P (2006) Applied microbiology and biotechnology in the conservation of stone culture heritage materials. Appl Microbiol Biotechnol 73:291–296CrossRefGoogle Scholar
  17. Ferris FG, Stehmeier LG (2002) US Patent 5143155. Bacteriogenic mineral plugging. E21B 043/22Google Scholar
  18. Ferris FG, Stehmeier LG, Kantzas A, Mourits FM (1996) Bacteriogenic mineral plugging. J Can Pet Technol 35:56–61Google Scholar
  19. Hammes F, Boon N, de Villiers J, Verstraete W, Siciliano SD (2003) Strain-specific ureolytic microbial calcium carbonate precipitation. Appl Environ Microbiol 69:4901–4909CrossRefGoogle Scholar
  20. Harkes MP, van Paassen LA, Booster JL, Whiffin VS, van Loosdrecht MCM (2010) Fixation and distribution of bacterial activity in sand to induce carbonate precipitation for ground reinforcement. Ecol Eng 36:112–117CrossRefGoogle Scholar
  21. Ivanov V (2010) Environmental microbiology for engineers. CRC Press, Boca Raton, p 438Google Scholar
  22. Ivanov V, Chu J (2008) Applications of microorganisms to geotechnical engineering for bioclogging and biocementation of soil in situ. Rev Environ Sci Biotechnol 7:139–153CrossRefGoogle Scholar
  23. Jin M, Rosario W, Walter E, Calhoun DH (2004) Development of a large-scale HPLC-based purification for the urease from Staphylococcus leei and determination of subunit structure. Protein Expr Purif 34:111–117Google Scholar
  24. Jonkers HM, Thijssen A, Muyzer G, Copuroglu O, Schlangen E (2010) Application of bacteria as self-healing agent for the development of sustainable concrete. Ecol Eng 36:230–235CrossRefGoogle Scholar
  25. Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, pp 115–175Google Scholar
  26. Li M, Cheng X, Guo H (2012) Heavy metal removal by biomineralization of urease producing bacteria isolated from soil. Int Biodeterior Biodegradation. doi: 10.1016/j.ibiod.2012.06.016 Google Scholar
  27. Lisdiyanti P, Suyanto E, Fahrurrozi RS, Sari MN, Gusmawati NF (2011) Bacterial carbonate precipitation for biogrouting. In: Proceedings of National Symposium on Ecohydrology Jakarta, pp 204–211Google Scholar
  28. Mitchell AC, Ferris FG (2005) The coprecipitation of Sr into calcite precipitates induced by bacterial ureolysis in artificial groundwater: temperature and kinetic dependence. Geochim Cosmochim Acta 69:4199–4210CrossRefGoogle Scholar
  29. Mortensen BM, DeJong JT (2011) Strength and stiffness of MICP treated sand subjected to various stress paths. In: Han J, Alzamora DA (eds) ASCE GeoFrontiers: Adv Geotech Eng 211:4012–4020Google Scholar
  30. Novakova D, Sedlacek I, Pantucek R, Stetina V, Svec P, Petras P (2006) Staphylococcus equorum and Staphylococcus succinus isolated from human clinical specimens. J Med Microbiol 55:523–528CrossRefGoogle Scholar
  31. Qian CX, Wang JY, Wang RX et al (2009) Corrosion protection of cement-based building materials by surface deposition of CaCO3 by Bacillus pasteurii. Mater Sci Eng C Biomim Supramol Syst 29:1273–1280. doi: 10.1016/j.msec.2008.10.025 CrossRefGoogle Scholar
  32. Ramachandran SK, Ramkrishnan V, Bang SS (2001) Remediation of concrete using microorganisms. ACI Mater J 98:3–9Google Scholar
  33. Rong H, Qian CX (2012) Development of microbe cementitious material in China. J Shanghai Jiaotong Univ (Sci) 17:350–355CrossRefGoogle Scholar
  34. Rong H, Qian CX, Li LZ (2012a) Influence of molding process on mechanical properties of sandstone cemented by microbe cement. Constr Build Mater 28:238–243CrossRefGoogle Scholar
  35. Rong H, Qian CX, Li LZ (2012b) Study on microstructure and properties of sandstone cemented by microbe cement. Constr Build Mater 36:687–694CrossRefGoogle Scholar
  36. Stabnikov V, Chu J, Naeimi M, Ivanov V (2011) Formation of water-impermeable crust on sand surface using biocement. Cem Concr Res 41:1143–1149CrossRefGoogle Scholar
  37. Taponen S, Björkroth J, Pyörälä S (2008) Coagulase-negative staphylococci isolated from bovine extramammary sites and intramammary infections in a single dairy herd. J Dairy Res 75:422–429CrossRefGoogle Scholar
  38. Tittelboom KV, De Belie N, De Muynck W, Verstraete W (2010) Use of bacteria to repair cracks in concrete. Cem Concr Res 40:157–166CrossRefGoogle Scholar
  39. van der Ruyt M, van der Zon W (2009) Biological in situ reinforcement of sand in near-shore areas. Geotech Eng 162:81–83CrossRefGoogle Scholar
  40. Whiffin VS (2004) Ph.D. Thesis, School of Biological Sciences and Biotechnology, Murdoch University, Perth, AustraliaGoogle Scholar
  41. Whiffin VS, van Paassen LA, Harkes MP (2007) Microbial carbonate precipitation as a soil improvement technique. Geomicrobiol J 24:17–423CrossRefGoogle Scholar
  42. Worrell E, Price L, Martin N, Hendriks C, Meida LO (2001) Carbon dioxide emissions from the global cement industry. Annu Rev Energy Environ 6:303–329CrossRefGoogle Scholar
  43. Zell C, Resch M, Rosenstein R, Albrecht T, Hertel C, Götz F (2008) Characterization of toxin production of coagulase-negative staphylococci isolated from food and starter cultures. Int J Food Microbiol 127:246–251CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Viktor Stabnikov
    • 1
    • 2
  • Chu Jian
    • 2
    • 3
  • Volodymyr Ivanov
    • 3
  • Yishan Li
    • 3
  1. 1.Department of Biotechnology and MicrobiologyNational University of Food TechnologiesKievUkraine
  2. 2.School of Civil and Environmental EngineeringNanyang Technological UniversitySingaporeSingapore
  3. 3.Department of Civil, Construction and Environmental EngineeringIowa State UniversityAmesUSA

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