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World Journal of Microbiology and Biotechnology

, Volume 29, Issue 2, pp 355–364 | Cite as

Isolation of proline-based cyclic dipeptides from Bacillus sp. N strain associated with rhabitid entomopathogenic nematode and its antimicrobial properties

  • Nishanth Kumar
  • C. Mohandas
  • Bala Nambisan
  • D. R. Soban Kumar
  • Ravi S. LankalapalliEmail author
Original Paper

Abstract

Entomopathogenic nematodes (EPN) are well-known as biological control agents and are found to have associated bacteria which can produce a wide range of bioactive secondary metabolites. We report herewith isolation of six proline containing cyclic dipeptides cyclo(d-Pro-l-Leu), cyclo(l-Pro-l-Met), cyclo(d-Pro-l-Phe), cyclo(l-Pro-l-Phe), cyclo(l-Pro-l-Tyr) and cyclo(l-Pro-d-Tyr) from ethyl acetate extract of the Luria Broth (LB) cell free culture filtrate of Bacillus sp. strain N associated with a new EPN Rhabditis sp. from sweet potato weevil grubs collected from Central Tuber Crops Research Institute farm. Antimicrobial studies of these 2,5-diketopiperazines (DKPs) against both medicinally and agriculturally important bacterium and fungi showed potent inhibitory values in the range of μg/mL. Cyclic dipeptides showed significantly higher activity than the commercial fungicide bavistin against agriculturally important fungi, viz., Fusarium oxysporum, Rhizoctonia solani, and Pencillium expansum. The highest activity of 2 μg/mL by cyclo(l-Pro-l-Phe) was recorded against P. expansum, a plant pathogen responsible for causing post harvest decay of stored apples and oranges. To our knowledge, this is the first report on the isolation of these DKPs from Rhabditis EPN bacterial strain Bacillus sp.

Keywords

Entomopathogenic nematodes Cyclic dipeptides Minimum inhibitory concentration Antimicrobial activity 

Notes

Acknowledgments

We are grateful to Dr. R. Srinivas, HOD, NCMS, IICT, Hyderabad for HRMS analysis. This work was funded by Indian council of Medical Research (ICMR).

Supplementary material

11274_2012_1189_MOESM1_ESM.doc (1.2 mb)
Supplementary data includes HRMS of DKPs and HPLC profile of FDAA derivatives of the acid hydrolysates of DKPs 1–6 and respective derivatives of l and d standard amino acids. (DOC 1186 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Nishanth Kumar
    • 1
  • C. Mohandas
    • 1
  • Bala Nambisan
    • 1
  • D. R. Soban Kumar
    • 2
  • Ravi S. Lankalapalli
    • 2
    Email author
  1. 1.Division of Crop Protection/Division of Crop UtilisationCentral Tuber Crops Research InstituteThiruvananthapuramIndia
  2. 2.Agroprocessing and Natural Products DivisionNational Institute for Interdisciplinary Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR)ThiruvananthapuramIndia

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