Actinomycetes from solitary wasp mud nest and swallow bird mud nest: isolation and screening for their antibacterial activity

  • Vijay Kumar
  • Alpana Bharti
  • Vivek Kumar Gupta
  • Omprakash Gusain
  • Gajraj Singh BishtEmail author
Original Paper


The aim of this study was to isolate and screen actinomycetes from solitary wasp and swallow bird mud nests for antimicrobial activity. The actinomycetes were isolated from soil of nests of solitary wasp and swallow bird, and identified on the basis of morphological characteristics and molecular biological methods. A total of 109 actinomycetal isolates were obtained from 12 soil samples (6 from each habitat) using two media. The highest number of actinomycetes were recovered on Humic acid vitamin agar media (65.13%, n = 71) as compared to actinomycetes isolation agar media (34.86%, n = 38). The antimicrobial activity of actinomycetes isolates was determined using the agar plug method. Among 109 isolates, 51 isolates (46.78%) showed antibacterial activity by agar plug assay. The morphological and molecular characteristics confirmed that the most of active isolates in both sample belonged to the genus Streptomyces, the other potential genera like Streptosporangium, Actinomadura, Saccharopolyspora, Thermoactinomycetes and Nocardia were also recovered, but in a low frequency. The isolates designated as 8(1)*, BN-6, MN 2(6), MN 2(7) and MN 9(V) showed most promising activity against various drug resistant bacterial pathogens. It seems that the promising isolates from these unusual/unexplored habitats may prove to be an important step in development of drug for treating multi-drug resistant bacterial pathogens.


Actinomycetes Antimicrobial activity Swallow bird mud nest Solitary wasp mud nest 



GRSB, VK and AB thank the Uttarakhand State Council of Science and Technology for grant received (UCS &T/R&D/LS/06-07/1158) to carry out this study and to the Management of S.B.S.P.G.I., Dehradun for providing necessary research facilities. The authors are also thankful to the Wadia Institute of Himalayan Geology, Dehradun, India for providing scanning electron microscopy facility.

Supplementary material

11274_2011_884_MOESM1_ESM.doc (997 kb)
Supplementary material 1 (DOC 997 kb)


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Vijay Kumar
    • 1
  • Alpana Bharti
    • 1
  • Vivek Kumar Gupta
    • 1
  • Omprakash Gusain
    • 2
  • Gajraj Singh Bisht
    • 1
    Email author
  1. 1.Department of MicrobiologySardar Bhagwan Singh Post Graduate Institute of Biomedical Sciences and ResearchDehradunIndia
  2. 2.Department of Zoology and BiotechnologyH.N.B. Garhwal UniversitySrinagarIndia

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