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World Journal of Microbiology and Biotechnology

, Volume 28, Issue 3, pp 1287–1294 | Cite as

An endophytic fungus from Azadirachta indica A. Juss. that produces azadirachtin

  • Souvik Kusari
  • Vijay C. Verma
  • Marc Lamshoeft
  • Michael SpitellerEmail author
Short Communication

Abstract

Azadirachtin A and its structural analogues are a well-known class of natural insecticides having antifeedant and insect growth-regulating properties. These compounds are exclusive to the neem tree, Azadirachta indica A. Juss, from where they are currently sourced. Here we report for the first time, the isolation and characterization of a novel endophytic fungus from A. indica, which produces azadirachtin A and B in rich mycological medium (Sabouraud dextrose broth), under shake-flask fermentation conditions. The fungus was identified as Eupenicillium parvum by ITS analysis (ITS1 and ITS2 regions and the intervening 5.8S rDNA region). Azadirachtin A and B were identified and quantified by LC-HRMS and LC-HRMS2, and by comparison with the authentic reference standards. The biosynthesis of azadirachtin A and B by the cultured endophyte, which is also produced by the host neem plant, provides an exciting platform for further scientific exploration within both the ecological and biochemical contexts.

Keywords

Endophytic fungus Neem Azadirachta indica Eupenicillium parvum Azadirachtin A Azadirachtin B 

Notes

Acknowledgments

We are grateful to the International Bureau of the German Federal Ministry of Education and Research (IB-BMBF/DLR), Germany, for supporting this work. We thank the Ministry of Innovation, Science, Research and Technology of the State of North Rhine-Westphalia, Germany, for financing a high-resolution mass spectrometer. V.C.V. acknowledges the fellowship from the German Academic Exchange Service (DAAD), Germany, for supporting this work.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Souvik Kusari
    • 1
  • Vijay C. Verma
    • 1
    • 2
  • Marc Lamshoeft
    • 1
  • Michael Spiteller
    • 1
    Email author
  1. 1.Faculty of ChemistryInstitute of Environmental Research (INFU)DortmundGermany
  2. 2.Centre of Experimental Medicine and Surgery, Institute of Medical SciencesBanaras Hindu UniversityVaranasiIndia

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