World Journal of Microbiology and Biotechnology

, Volume 28, Issue 2, pp 485–491 | Cite as

Bactericidal activity of ethanolic extracts of propolis against Staphylococcus aureus isolated from mastitic cows

  • Henrique Freitas Santana
  • Ana Andréa Teixeira Barbosa
  • Sukarno Olavo Ferreira
  • Hilário Cuquetto Mantovani
Original Paper

Abstract

Staphylococcus aureus is an important pathogen for both humans and animals, and it has been an ubiquitous etiological agent of bovine mastitis in dairy farms worldwide. Elimination of S. aureus with classic antibiotics is difficult, and the current study aimed to evaluate the efficacy of ethanolic extracts of propolis (EEP) against S. aureus cultivated in complex media or milk. EEP (0–0.5 mg ml−1) decreased growth of S. aureus in BHI media and 1 mg ml−1 was bactericidal against washed cell suspensions (107 CFU ml−1). Propolis extracts also killed S. aureus cells resuspended in milk, but the bactericidal dose was at least 20-fold greater. Cultures that were transferred for at least 60 generations with sub-lethal doses of propolis did not change much their sensibility to EEP. Atomic force microscopy images revealed changes in morphology and cell size of S. aureus cells exposed to EEP (0.5 mg ml−1). Our results indicate that propolis extracts might be effective against mastitis-causing S. aureus strains in vivo, but milk constituents affect the inhibitory activity of propolis. Considering that propolis-resistance appears to be a phenotype not easily selected, the use of EEP combined or not with other antimicrobial agents might be useful for mastitis control in vivo.

Keywords

Baccharis dracunculifolia Mastitis Udder infection AFM Dairy 

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Henrique Freitas Santana
    • 1
  • Ana Andréa Teixeira Barbosa
    • 1
  • Sukarno Olavo Ferreira
    • 2
  • Hilário Cuquetto Mantovani
    • 1
  1. 1.Departamento de MicrobiologiaUniversidade Federal de ViçosaViçosa-MGBrazil
  2. 2.Departamento de FísicaUniversidade Federal de ViçosaViçosa-MGBrazil

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