An effective and simplified scale-up strategy for acarbose fermentation based on the carbon source control
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The scale-up strategy for acarbose fermentation by Actinoplanes sp. A56 was explored in this paper. The results obtained in shake-flask cultivation demonstrated that the ratio of maltose and glucose had significant effects on the biosynthesis of acarbose, and the feeding medium containing 3:1 (mass ratio) of maltose and glucose was favorable for acarbose production. Then the correlation of the carbon source concentration with acarbose production was further investigated in 100-l fermenter, and the results showed that 7.5–8.0 g of total sugar/100 ml and 4.0–4.5 g of reducing sugar/100 ml were optimal for acarbose production. Based on the results in 100-l fermenter, an effective and simplified scale-up strategy was successfully established for acarbose fermentation in a 30-m3 fermenter, by using total sugar and reducing sugar as the scale-up parameter. As a result, 4,327 mg of acarbose/l was obtained at 168 h of fermentation.
KeywordsActinoplanes sp. A56 Acarbose production Carbon source Scale-up
This work was supported by Jiangxi Key Technology R&D Program (2009BGA02100) and JXAU Scientific Research Foundation for Doctor (2008–2064). The authors gratefully acknowledge the Ministry of Science and Technology of Jiangxi Province.
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