Biosafety evaluation of bacteriophages for treatment of diarrhea due to intestinal pathogen Escherichia coli 3-2 infection of chickens
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Intestinal Escherichia coli caused diarrhea in chicken makes serious damage directly to the chicken culture industry. Bacteriophage therapy is able to control the diarrhea in chickens effectively. In this study, the biosafety of bacteriophages was evaluated for treating intestinal pathogenic E.coli, which induced diarrhea in chickens. Ten bacteriophages were isolated from feces of chickens with diarrhea using the ill-chicken intestinal pathogenic E. coli 3-2 as target organism. Three bacteriophages propagated on E. coli 3-2 with relative big and clear plaques were selected and used together for toxicity experiment and evaluating the effect of therapy on chicken weight gain. In 3 weeks of trial, no mice given with or without mixed bacteriophages died, and the weight of mice of the experimental group did not show significant difference to the control group after 3 weeks infection. Besides remarkable decreasing the death rate of chickens with diarrhea, treatment of mixed bacteriophages also promoted the weight gain and saved the diet consumption as the utilize rate of diet increased 11% compared with the control group. These observations indicated that a mixture of three bacteriophages would be biosafe for rapid and effective preventing pathogenic E. coli infections.
KeywordsDiarrhea Pathogenic Escherichia coli Baceriophage Biosafety
This work was supported by the National Science Foundation of China (NSFC, Project No. 30200004).
Conflict of interest
The authors declare that they have no conflict of interest.
- Adams MH (ed) (1959) Enumeration of bacteriophage particles. In: Bacteriophages. Interscience Publishers, NY, pp 27–30Google Scholar
- Barrow PA (1986) Bacteriophages mediating somatic antigenic conversion in Salmonella cholerae-suis; their isolation from sewage and other Salmpnella serotypes possessing the somatic 6 antigen. J Gen Microbiol 132:835–837Google Scholar
- Barrow P, Lovell M, Berchieri A Jr (1998) Use of lytic bacteriophage for control of experimental Escherichia coli septicemia and meningitis in chickens and calves. Clin Diagn Lab Immunol 5:294–298Google Scholar
- Carlton RM (1999) Phage therapy: past history and future prospects. Arch Immunol Ther Exp (Warsz) 47:267–274Google Scholar
- D’Herelle F (1917) Sur un microbe invisible antagonists des bacilles dysenteriques. Comptes Rendus Acad Sci Paris 165:373–375Google Scholar
- Doyle MP, Erickson MC (2006) Reducing the carriage of foodborne pathogens in livestock and poultry. Poult Sci 85:960–973Google Scholar
- Gorski A, Miedzybrodzki R, Borysowski J, Weber-Dabrowska B, Lobocka M, Fortuna W, Letkiewicz S, Zimecki M, Filby G (2009) Bacteriophage therapy for the treatment of infections. Curr Opin Investig Drugs 10:766–774Google Scholar
- Huff WE, Huff GR, Rath NC, Balog JM, Donoghue AM (2002a) Prevention of Escherichia coli infection in Broiler chickens with a bacteriophage aerosol spray. Poult Sci 81:1486–1491Google Scholar
- Huff WE, Huff GR, Rath NC, Balog JM, Xie H, Moore PA, Donoghue AM (2002b) Prevention of Escherichia coli respiratory infection in Broiler chickens with bacteriophage (SPRO2). Poult Sci 81:437–441Google Scholar
- Inal JM (2003) Phage therapy: a reappraisal of bacteriophages as antibiotics. Arch Immunol Ther Exp (Warsz) 51:237–244Google Scholar
- Jagusztyn-Krynicka EK, Wyszyńska A (2008) The decline of antibiotic era–new approaches for antibacterial drug discovery. Pol J Microbiol 57:91–98Google Scholar
- Li H, Xie HJ, Kong J, Ma GR (2004) Biological characterization of fowl intestinal bacteriophage. Microbiology 31:10–13 (in Chinese)Google Scholar
- Oliveira A, Sereno R, Azeredo J (2010) In vivo efficiency evaluation of a phage cocktail in controlling severe colibacillosis in confined conditions and experimental poultry houses. Vet Microbiol 146:303–308Google Scholar
- Smith HW, Huggins MB (1982) Successful treatment of experimental Escherichia coli infections in mice using phage: its general superiority over antibiotics. J Gen Microbiol 128:307–318Google Scholar
- Smith HW, Huggins MB (1983) Effectiveness of phages in treating experimental Escherichia coli diarrhoea in calves, piglets and lambs. J Gen Microbiol 129:2659–2675Google Scholar
- Weber-Dabrowska B, Mulczyk M, Gorski A (2000) Bacteriophage therapy of bacterial infections: an update of our institute’s experience. Arch Immunol Ther Exp (Warsz) 48:547–551Google Scholar