Green synthesis of silver nanoparticles using Rhodobacter Sphaeroides
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The use of microorganisms in the synthesis of nanoparticles emerges as an eco-friendly and exciting approach. In this study, silver nanoparticles were successfully synthesized from AgNO3 by reduction of aqueous Ag+ ions with the cell filtrate of Rhodobacter sphaeroides. Nanoparticles were characterized by means of UV–vis absorption spectroscopy, X-Ray Diffraction (XRD), transmission electron microscopy (TEM) and high-resolution transmission electron microscopy (HRTEM). Crystalline nature of the nanoparticles in the fcc structure are confirmed by the peaks in the XRD pattern corresponding to (111), (200), (220) and (311) planes, bright circular spots in the selected are a electron diffraction (SAED) and clear lattice fringes in the high-resolution TEM image. Also, the size of silver nanoparticles was controlled by the specific activity of nitrate reductase in the cell filtrate.
KeywordsRhodobacter sphaeroides Biosynthesis Silver nanoparticles Cell filtrate
The authors gratefully acknowledge the financial supports from the Shanxi Province Postdoctoral Science Foundation, the Provincial Key Technology R&D Program of Shanxi Province, China (No. 20080311027-1), the Eighth Youth Innovation Science Fund Program of China North Industries Group Corportion.
- Chen JH, Tao L, Li J (2006) Biochemistry laboratory, 6th ed. Science Press, Beijing, pp 63–64 (in Chinese)Google Scholar
- Kerber NL, Cardenas J (1982) Nitrate reductase from Rhodopseudomonas sphaeroides. J Bacteriol 153:1091–1097Google Scholar
- Kessi J, Ramuz M, Wehrli E, Spycher M, Bachofen R (1999) Reduction of selenite and detoxification of elemental selenium by the phototrophic bacterium Rhodospirillum rubrum. Appl Environ Microb 65:4734–4740Google Scholar
- Kobayashi M, Kurata S (1978) The mass culture and cell utilization of photosynthetic bacteria. Process Biochem 13:27–29Google Scholar
- Li G, Jiao R (1995) Nitrate assimilation of amcolatopsis mediterrandi U-32 and some properties of its nitrate reductase. Acta Microbiol Sinica 35:111–118Google Scholar
- Mokhtari N, Daneshpajouh S, Seyedbagheri S, Atashdehghan R, Abdi K, Sarkar S, Minaian S, Shahverdi HR, Shahverdi AR (2009) Biological synthesis of very small silver nanoparticles by culture supernatant of Klebsiella pneumonia: the effects of visible-light irradiation and the liquid mixing process. Mater Res Bull 44:1415–1421CrossRefGoogle Scholar
- Mukherjee P, Ahmad A, Mandal D, Senapati S, Sainkar SR, Khan MI, Ramani R, Parischa R, Ajaykumar PV, Alam M, Sastry M, Kumar R (2001) Bioreduction of AuCl4− ions by the Fungus, Verticillium sp. and surface trapping of the gold nanoparticles formed. Angew Chem Int Ed 40:3585–3588CrossRefGoogle Scholar
- Yao ZY, Zhang ZM (1996) Phenotypic features and DNA–DNA homology analyses of some photosynthetic bacteria. Chin J Appl Environ Biol 2:84–89 (in Chinese)Google Scholar