Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Purification and characterization of a novel poly(butylene succinate)-degrading enzyme from Aspergillus sp. XH0501-a

  • 200 Accesses

  • 5 Citations


A poly(butylene succinate) (PBS)-degrading Aspergillus sp. XH0501-a was obtained by ultraviolet light compound LiCl mutagenesis from the Aspergillus sp. XH0501 which was isolated from soil. The enzymatic activity of strain XH0501-a was 38.89% higher than that of the wild-type strain. A novel extracellular PBS-degrading enzyme with a molecular weight of 44.7 kDa was purified to homogeneity from the culture supernatant of XH0501-a strain. The optimum temperature and pH for the enzyme activity was 40°C and pH 8.6, respectively. It was found that Fe2+ and Ca2+ enhanced the enzyme activity, whereas Cu2+ and Hg2+ inhibited it. The primary products after enzymatic degradation were identified by mass spectrometric analysis and the results indicated that the enzyme was of the exo-type and cut the chain from the carboxyl end; the affinity for the substrate was relative to the chain length of the carboxylic ester.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5


  1. Auras R, Harte B, Selke S (2004) An overview of polylactides as packaging materials. Macromol Biosci 4:835–864

  2. Chen S, Liu DB, Xia HM, He MY, Zhuang YG, Dong LS (2002) Purification and properties of poly (β-hydroxybutyrate) depolymerase. J Mol Sci (China) 18:220–225

  3. Ci SQ, Chen S, Liu DB, Xia HM (2006) An extracellular poly (3-hydroxybutyrate) depolymerase from Penicillium sp. DS9713a–01. World J Microbiol Biotechnol 22:729–735

  4. Giorgio M (2000) Synthesis and enzymatic degradation of aliphatic copolyesters. Polym Degrad Stab 70:305–310

  5. Gross RA, Kalra B (2002) Biodegradable polymers for the environment. Science 297:803–807

  6. Hayase N, Yano H, Kudoh E, Tsutsumi C, Ushio K, Miyahara Y, Tanaka S, Nakagawa K (2004) Isolation and characterization of poly(butylene succinate-co-butylene adipate)-degrading microorganism. J Biosci Bioeng 97:131–133

  7. Hoshino A, Isono Y (2002) Degradation of aliphatic polyester films by commercially available lipases with special reference to rapid and complete degradation of poly(L-lactide) film by lipase PL derived from Alcaligenes sp. Biodegradation 13:141–147

  8. Jarerat A, Tokiwa Y, Tanaka H (2006) Production of poly(L-lactide)-degrading enzyme by Amycolatopsis orientalis for biological recycling of poly(L-lactide). Appl Microbiol Biotechnol 72:726–731

  9. Kim MN, Kim KH, Jin HJ (2001) Biodegradability of ethyl and n-octyl branched poly(ethylene adipate) and poly(butylene succinate). Eur Polym J 37:1843–1847

  10. Maeda H, Yamagata Y, Abe K, Hasegawa F, Machida M, Ishioka R, Gomi K, Nakajima T (2005) Purification and characterization of a biodegradable plastic-degrading enzyme from Aspergillus oryzae. Appl Microbiol Biotechnol 67:778–788

  11. Marija SN, Jasna D (2001) Synthesis and characterization of biodegradable poly(butylenes succinate-co-butylene adipate)s. Polym Degrad Stab 74:263–270

  12. Nishida H, Tokiwa Y (1993) Distribution of poly(-hydroxybutyrate) and poly(ε-caprolactone) aerobic degrading microorganisms in different environments. J Environ Polym Degrad 1:227–233

  13. Tokiwa Y, Calabia BP (2006) Biodegradability and biodegradation of poly(L-lactide). Appl Microbiol Biotechnol 72:244–251

  14. Tomita K, Kuroki Y, Hayashi N, Komukai Y (2000) Isolation of a thermophile degrading poly(butylene succinate-co-butylene adipate). J Biosci Bioeng 90:350–352

  15. Tserki V, Matzinos P, Pavlidou E (2006) Biodegradable aliphatic polyesters. Part I. Properties and biodegradation of poly(butylene succinate-co-butylene adipate). Polym Degrad Stab 91:367–376

  16. Wei JC (1979) Fungus handbook. Shanghai Science & Technology Press, Shanghai

  17. Zhang MC (1984) Industrial micro breeding. Beijing Science Press, Beijing

  18. Zhao JH, Wang XQ, Zeng J (2004) Study on the biodegradation of poly(butylene succinate) under compost conditions. J Function Polymers (China) 17:666–670

Download references


This work was supported by the National Natural Science Foundation of China (J0830627-2), the Development Program of Jilin Province (20090594) and the Fundamental Research Funds for the Central Universities (09QNJJ019).

Author information

Correspondence to Shan Chen.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Li, F., Hu, X., Guo, Z. et al. Purification and characterization of a novel poly(butylene succinate)-degrading enzyme from Aspergillus sp. XH0501-a. World J Microbiol Biotechnol 27, 2591–2596 (2011).

Download citation


  • Poly(butylene succinate)
  • Aspergillus sp. XH0501-a
  • PBS-degrading enzyme
  • Exo-type