World Journal of Microbiology and Biotechnology

, Volume 27, Issue 10, pp 2305–2313 | Cite as

Phenotypic and molecular characterization of rhizobacterium Burkholderia sp. strain R456 antagonistic to Rhizoctonia solani, sheath blight of rice

Original Paper
First Online:
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Abstract

The effect of rhizobacterium Burkholderia sp. strain R456 on the inhibition of Rhizoctonia solani, sheath blight of rice was examined. Results from this study indicated that strain R456 not only suppressed the in vitro mycelial growth of R. solani, but also reduced the incidence and severity of rice sheath blight under greenhouse conditions. However, similar to plant pathogenic strain LMG 1222T of Burkholderia cepacia, the type species of the genus, infiltration of tobacco leaves with cell suspension of strain R456 resulted in typical hypersensitivity reactions while the two bacterial strains were unable to cause disease symptoms on rice seedlings. The fatty acid methyl ester profile, sole carbon source utilization, and biochemical tests confirmed that the antagonistic rhizobacterium R456 is a member of the genus Burkholderia. Furthermore, strain R456 was differentiated from B. cepacia LMG 1222T and was identified as Burkholderia seminalis based on recA gene sequence analysis and multilocus sequence typing. In addition, this rhizobacterium had a lower proteolytic activity compared with that of the pathogenic B. cepacia LMG 1222T while no cblA and esmR marker genes were detected for the two bacterial strains. Overall, this is the first characterization of rhizobacterium B. seminalis that protected rice seedlings from infection by R. solani.

Keywords

Antagonism Burkholderia seminalis Rhizoctonia solani Rice sheath blight Characterization 
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Notes

Acknowledgments

This project was supported by Zhejiang Provincial Natural Science Foundation of China (Y3090150), the Fundamental Research Funds for the Central Universities (KYJD09022), Zhejiang Provincial Project (2010R10091), the Agricultural Ministry of China (nyhyzx07-056; nyhyzx200803010) and Specialized Research Fund for the Doctoral Program of Higher Education (20090101120083).

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.State Key Laboratory of Rice Biology, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of BiotechnologyZhejiang UniversityHangzhouChina
  2. 2.Zhejiang University of TechnologyHangzhouChina
  3. 3.State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest and Disease Control, Institute of Plant Protection MicrobiologyZhejiang Academy of Agricultural SciencesHangzhouChina

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