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Studies on the ecology of actinomycetes in an agricultural soil amended with organic residues: II. Assessment of enzymatic activities of Actinomycetales isolates

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Abstract

The main objective of this investigation was to study the enzymatic activities of Actinomycetales strains isolated from an agricultural soil amended with different amounts of municipal solid waste compost (MSWC) or farmyard manure (FM). For this purpose, the hydrolytic activities of carboxymethyl cellulase, xylanase, pectinase, amylase, chitinase and protease were tested for 75 isolates of Sterptomyces, Amycolatopsis and Nocardioides from different sources (unamended soil, amended soil with FM or MSWC, FM and MSWC) at temperature ranging between 30 and 50°C. It was shown that the highest rate of enzymes producer’s strains was registered at 30°C, and decreased gradually to annul at 50°C, with the exception of the MSWC strains origin. It was also shown that the percentage of strains producers of enzymes isolated from soil amended with MSWC appeared higher than the one registered for those isolated from control and amended with FM soils. Application of MSWC increases the number of enzymes producer-actinomycetes in the soil and then it improves its fertility.

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Acknowledgments

The present study is a part of the 1999–2006 research program ‘‘Municipal solid waste treatment and compost agricultural re-use’’ which is financially by the Tunisian State Secretariat of Scientific Research and Technology.

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Correspondence to Mokni-Tlili Sonia.

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Sonia, M., Hafedh, B., Abdennaceur, H. et al. Studies on the ecology of actinomycetes in an agricultural soil amended with organic residues: II. Assessment of enzymatic activities of Actinomycetales isolates. World J Microbiol Biotechnol 27, 2251–2259 (2011). https://doi.org/10.1007/s11274-011-0688-4

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Keywords

  • Actinomycetales
  • Soil amendment
  • MSW compost
  • Farmyard manure
  • Hydrolytic activities