World Journal of Microbiology and Biotechnology

, Volume 27, Issue 9, pp 2139–2146 | Cite as

Kinetics and physico-chemical characterization of exopolysaccharides produced by the cyanobacterium Oscillatoria formosa

Original Paper
First Online:
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Accepted:

Abstract

Exopolysaccharides of the cyanobacterium Oscillatoria formosa have been physico-chemically characterized and kinetics of their production studied. The organism produced 334.8 μg EPS per ml culture in 24 days with the maximum rate of production obtained during initial days of growth. HPLC analysis of the EPS hydrolysate revealed that besides three unidentified sugars, EPS contained ribose, mannose, and galacturonic acid. FT-IR spectrum of EPS revealed the presence of methyl, carboxyl and C–N groups. Elemental analysis indicated the presence of 4.7% nitrogen in EPS. The organism produced 75.6% more EPS when incubated at 35°C compared to cultures at 28°C. Under varied nutritional conditions, though the growth of the organism was less yet it produced enhanced amounts of EPS. Aqueous dispersions of EPS showed non-Newtonian, pseudoplastic behaviour. The viscosity of the aqueous solution of EPS was quite stable over a wide range of pH and temperature but it was observed to be affected by CaCl2.

Keywords

Cyanobacterium Exopolysaccharides Kinetics Physico-chemical characterization Oscillatoria formosa Rheology 
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Notes

Acknowledgments

The authors are thankful to the Head, Department of Botany, Punjabi University, Patiala for laboratory facilities. Financial assistance from Council of Scientific & Industrial Research, New Delhi in the form of a research scheme No. 38(1189)/08/EMR-II is gratefully acknowledged.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Department of BotanyPunjabi UniversityPatialaIndia

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