Phylogenetic diversity of bacteria associated with the endemic freshwater sponge Lubomirskia baicalensis
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In this study, for the first time the diversity of bacteria associated with the endemic freshwater sponge Lubomirskia baicalensis collected from the Sousern Basin of Lake Baikal was investigated employing cultivation-independent approaches. In total, 102 bacterial 16S rRNA clones were screened using restriction fragment length polymorphism (RFLP) and 30 were selected for sequencing. BLASTN and phylogenetic analysis based on near full length 16S rDNA sequences showed that 22 operational taxonomic units (OTUs) were clustered in six known phyla: Actinobacteria (8 OTUs), alpha-Proteobacteria (4 OTUs), beta-Proteobacteria (4 OTUs), Verrucomicrobia (4 OTUs), Nitrospiracea (1 OTU) and Bacteroidetes (1 OTU). Remarkably all phylotypes were affiliated to uncultured microorganisms, however, all alpha-Proteobacteria sequences were closely related to bacteria derived from the freshwater sponge Spongilla lacustris. Our results reveal a high diversity in the L. baicalensis bacterial community and provide an insight into microbial ecology and diversity within freshwater sponges inhabiting the ancient Lake Baikal ecosystem.
KeywordsPorifera Freshwater sponge Lubomirskia baicalensis Microbial diversity 16S rRNA
This study was funded by Yong Scientist Lavrentiev’s Grant No. 6 provided by Siberian Branch of Russian Academy of Science.
- Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410Google Scholar
- Efremova SM (2001) Sponges (Porifera). In: Timoshkin OA (ed) Index of animal species inhabiting Lake Baikal and its catchment area. Nauka, Novosibirsk, pp 182–192Google Scholar
- Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
- Manconi R, Pronzato R (2002) Suborder Spongillina subord. nov.: freshwater sponges. In: Hooper NJA, van Soest RWM (eds) Systema Porifera: a Guide to the Classification of Sponges. Kluwer Academic/Plenum Publisher, New York, pp 921–1019Google Scholar
- Timoshkin OA (ed) (1995) Index of animal species inhabiting Lake Baikal and its catchement area. Nauka, Novosibirsk, pp 1261–1278Google Scholar
- Zhu P, Li Q, Wang G (2008) Unique microbial signatures of the alien Hawaiian marine sponge Suberites zeteki. Microb Ecol 55: 406–414Google Scholar