Development of IRAP-SCAR marker for strain identification in Lentinula edodes

  • Yang Xiao
  • Yaohong Dai
  • Yingying Lu
  • Wei Liu
  • Zhuoren Wang
  • Yinbing Bian
Short Communication
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Abstract

Rapid and correct authentication of commercial strains is still important in today’s mushroom industry. Here for the first time we reported the using of sequence characterized amplified region (SCAR) marker developed from inter-retrotransposon amplified polymorphism (IRAP) marker to identify Lentinula edodes strain. Genomic polymorphism among 44 shiitake cultivars in China were surveyed by 24 IRAP primer combinations, from which primer combination LTR1L/MarY1R could generate a unique and reproducible 1712 bp fragment to distinguish strain No. 4 from other 43 strains. Based on this strain-specific fragment, a SCAR primer pair was designed and its validity was verified by correctly amplifying a single strain-specific fragment from DNA samples of 100 L. edodes strains. Our study lays the foundation for developing strain-specific SCAR marker by retrotransposon-based marker technique in fungi.

Keywords

Shiitake IRAP marker SCAR marker Strain identification 
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Notes

Acknowledgments

This work was financially supported by the National Key Technology R&D Program in the 11th Five-year Plan of China (Grant No. 2008BADA1B02), and the Industry (Agriculture), Science and Technology Plans of China (Grant No. nyhyzx07-008).

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Yang Xiao
    • 1
  • Yaohong Dai
    • 1
  • Yingying Lu
    • 1
  • Wei Liu
    • 1
  • Zhuoren Wang
    • 1
  • Yinbing Bian
    • 1
  1. 1.Institute of Applied MycologyHuazhong Agricultural UniversityWuhanChina

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