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Effects of different nitrogen rich substrates and their combination to the yield performance of oyster mushroom (Pleurotus ostreatus)

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Abstract

This study was conducted to assess the effect of nitrogen (N) variations from lignocellulosic substrates of empty fruit bunch (EFB), palm oil frond (POF), palm oil trunks (POT), A.mangium bark (AB) and Cogongrass (IC), and combinations among them using biological supplements (POF and POT) for suitability as a substrate of P. ostreatus. Three different sets of substrates were evaluated in response to different parameters of P. ostreatus including spawn running, primordial initiation, fruit body yield and biological efficiency (BE). The supplementation of POT and POF generally revealed significant differences on the crop performance of P. ostreatus compared to the un-supplemented substrate. POT demonstrated reduced production time, while POF was the best supplement to promote fresh weight and BE. The most rapid spawn running took place within 18.6 ± 0.9 days, while the primordial initiation was observed within 23.2 ± 0.5 days in the combination of IC + POT. The most suitable substrate for higher mushroom weight and BE was IC + POF (19.6 ± 1.3 g; 70.5 ± 5.5%). In addition, the interval between primordial formations was found to be fastest in the un-supplemented EFB and the combination of EFB + POF, which supported a shortened production time. Therefore, IC and EFB appeared to be the better alternative materials for growing P. ostreatus, either with or without any supplementation.

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Acknowledgments

The authors would like to express their deep gratitude to PT. ASTRA and PT. INHUTANI I for providing the research material. Further appreciation is extended to the Laboratory of Wood Chemistry of Mulawarman University; Dipterocarp Research Center (DiReC) of Kalimantan; and the Laboratory of Forest Product, Forest Product Research Institute of Asahikawa, Hokkaido, Japan, which have provided valuable support during the laboratory work. Also, special gratitude goes to Eliya, Uus and Reza for their fieldwork during data collection. Financial support from the Japan Society for the Promotion of Science (JSPS #19255016) is gratefully acknowledged. The authors also appreciate Yaya Rayadin, Roberto Delgado, Stephanie Spehar and Eleanor Clark for critical scrutiny on the earlier draft of this scientific writing.

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Authors and Affiliations

  1. Laboratory of Forest Resource Biology, Division of Environmental Resources, Graduate School of Agriculture, Hokkaido University, N-9, W-9, Kita-ku, Sapporo, Hokkaido, 060-8589, Japan

    Maharani Rizki & Yutaka Tamai

  2. Dipterocarp Research Center (DiReC), Forest Research Development Agency, Ministry of Forestry, Jalan A.W. Syahrani No.68, Samarinda, East Kalimantan, 75119, Indonesia

    Maharani Rizki

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  1. Maharani Rizki
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Correspondence to Maharani Rizki.

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Rizki, M., Tamai, Y. Effects of different nitrogen rich substrates and their combination to the yield performance of oyster mushroom (Pleurotus ostreatus). World J Microbiol Biotechnol 27, 1695–1702 (2011). https://doi.org/10.1007/s11274-010-0624-z

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