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Lignin biodegradation and ligninolytic enzyme studies during biopulping of Acacia mangium wood chips by tropical white rot fungi

  • C. Y. Liew
  • A. HusainiEmail author
  • H. Hussain
  • S. Muid
  • K. C. Liew
  • H. A. Roslan
Original Paper

Abstract

White rot fungi are good lignin degraders and have the potential to be used in industry. In the present work, Phellinus sp., Daedalea sp., Trametes versicolor and Pycnoporus coccineus were selected due to their relatively high ligninolytic enzyme activity, and grown on Acacia mangium wood chips under solid state fermentation. Results obtained showed that manganese peroxidase produced is far more compared to lignin peroxidase, suggesting that MnP might be the predominating enzymes causing lignin degradation in Acacia mangium wood chips. Cellulase enzyme assays showed that no significant cellulase activity was detected in the enzyme preparation of T. versicolor and Phellinus sp. This low cellulolytic activity further suggests that these two white rot strains are of more interest in lignin degradation. The results on lignin losses showed 20–30% of lignin breakdown at 60 days of biodegradation. The highest lignin loss was found in Acacia mangium biotreated with T. versicolor after 60 days and recorded 26.9%, corresponding to the percentage of their wood weight loss recorded followed by P. coccineus. In general, lignin degradation was only significant from 20 days onwards. The overall percentage of lignin weight loss was within the range of 1.02–26.90% over the biodegradation periods. Microscopic observations conducted using scanning electron microscope showed that T. versicolor, P. coccineus, Daedalea sp. and Phellinus sp. had caused lignin degradation in Acacia mangium wood chips.

Keywords

Lignin peroxidase Manganese peroxidase Laccase Cellulase Ligninolytic Biopulping 

Notes

Acknowledgments

This project was funded by the UNIMAS Fundamental Research Grant: 01(133)/530/2005(29). Authors are thankful to the personnel at Timber Research & Technical Training Centre, Sarawak Forestry Corporation (SFC) for providing technical advice and facilities for this work.

References

  1. Akhtar M, Blanchette R, Kirk TK (1997) Fungal delignification and biomechanical pulping of wood. In: Eriksson K-EL et al (eds) Biotechnology in the pulp and paper industry (Advances in Biochemical Engineering Biotechnology). Springer, Berlin/Heidelberg, pp 159–195CrossRefGoogle Scholar
  2. Akhtar M, Blanchette RA, Myers GC, Kirk TK (1998) An overview of biochemical pulping research. In: Young R, Akhtar M (eds) Environmentally friendly technologies for the pulp and paper industry. Wiley, New York, pp 309–340Google Scholar
  3. Akhtar M, Scott GM, Swaney RE, Shipley DF (2000) Biomechanical pulping a mill-scale evaluation. Resour Conserv Recycl 28:241–252CrossRefGoogle Scholar
  4. Ali M, Sreekrishnan TR (2001) Aquatic toxicity from pulp and paper mill effluents: a review. Adv Environ Res 5(2):175–196CrossRefGoogle Scholar
  5. Archibald F (1992) A new assay for lignin-type peroxidases employing the dye azure B. Appl Environ Microbiol 58:3110Google Scholar
  6. Asgher M, Asad MJ, Legge RL (2006) Enhanced lignin peroxidase synthesis by Phanerochaete chrysosporium in solid state bioprocessing of a lignocellulosic substrate. World J Microbiol Biotechnol 22(5):445–449CrossRefGoogle Scholar
  7. Barr DP, Aust SD (1994) Conversion of lignin peroxidase compound III to active enzyme by cation radicals. Arch Biochem Biophys 312(2):511–515Google Scholar
  8. de Souza-Cruz P, Freer J, Siika-Aho M, Ferraz A (2004) Extraction and determination of enzymes produced by Ceriporiopsis subvermispora during biopulping of Pinus taeda wood chips. Enzyme Microb Technol 34:228–234CrossRefGoogle Scholar
  9. Dittmer JK, Patel NJ, Dhawale SW, Dhawale SS (1997) Production of multiple laccase isoforms by Phanerochaete chrysosporium grown under nutrient sufficiency. FEMS Microbiol Lett 149:65–70CrossRefGoogle Scholar
  10. Eaton RA, Hale MDC (1993) Wood: decay, pests, and protection. Chapman & Hall, LondonGoogle Scholar
  11. Enoki M, Watanabe T, Nakagame S, Koller K, Messner K, Honda Y, Kuwahara M (1999) Extracellular lipid peroxidation of selective white rot fungus, Ceriporiopsis subvermispora. FEMS Microbiol Lett 180:205–211CrossRefGoogle Scholar
  12. Eriksson K-EL, Blanchette RA, Ander P (1990) Microbial and enzymatic degradation of wood and wood components. Springer, BerlinGoogle Scholar
  13. Eveleigh D, Mandels M, Andreotti R, Roche C (2009) Measurement of saccharifying cellulase. Biotechnol Biofuels 2:21CrossRefGoogle Scholar
  14. Ferraz A, Córdova A, Machuca A (2003) Wood biodegradation and enzyme production by Ceriporiopsis subvermispora during solid-state fermentation of Eucalyptus grandis. Enzyme Microb Technol 32:59–65CrossRefGoogle Scholar
  15. Ferraz A, Guerra A, Mendonca R, Masarin F, Vicentim MP, Aguiar A, Pavan PC (2008) Technological advances and mechanistic basis for fungal biopulping. Enzyme Microb Technol 43:178–185CrossRefGoogle Scholar
  16. Gianna P, Paola G, Carmen B, Andrea S, Antonio C, Giovanni S (1997) A novel white laccase from Pleurotus ostreatus. J Biol Chem 272(50):31301–31307Google Scholar
  17. Haddadin M, Al-Natour R, Al-Qsous S, Robinson R (2002) Bio-degradation of lignin in olive pomace by freshly-isolated species of Basidiomycete. Bioresour Technol 82:131–138CrossRefGoogle Scholar
  18. Hammel KE (1997) Fungal degradation of lignin. In: Cadisch G, Giller KE (eds) Driven by nature: plant litter quality and decomposition. CAB International, Wallingford, pp 33–46Google Scholar
  19. Itoh H, Wada M, Honda Y, Kuwahara M, Watanabe T (2003) Bioorganosolve pretreatments for simultaneous saccharification and fermentation of beech wood by ethanolysis and white rot fungi. J Biotechnol 103:273–280CrossRefGoogle Scholar
  20. Keller FA, Hamilton JE, Nguyen QA (2003) Microbial pretreatment of biomass potential for reducing severity of thermo-chemical biomass pretreatment. Appl Biochem Biotechnol 105:27–41CrossRefGoogle Scholar
  21. Muzariri C, Mazorodze JH, Mapingire J (2002) Spectrophotometric monitoring of the decolourisation of pulp and paper effluent water and the simultaneous production of lignolytic enzymes. In: 3rd WATERNET/WARFSA symposium “Water Demand Management for Sustainable Development”, Dar es Salaam, Tanzania. 30–31 OctoberGoogle Scholar
  22. Paszczynski A, Crawford R, Huynh V (1988) Manganese peroxidase of Phanerochaete chrysosporium: purification. Methods Enzymol 161:264–270CrossRefGoogle Scholar
  23. Reid ID (1998) Bleaching kraft pulps with white-rot fungus. In: Young RA, Akhtar M (eds) Environmentally friendly technologies for the pulp and paper industry. Wiley, New York, pp 505–514Google Scholar
  24. Shukla OP, Rai UN, Subramanian SV (2004) Biopulping and biobleaching. An energy and environment saving technology for Indian pulp and paper industry. Newslett ISEB India 10(2): AprilGoogle Scholar
  25. Singh A, Wong A, Kim Y, Wi S, Lee K (2003) Soft rot decay of cengal (Neobalanocarpus heimii) heartwood in ground contact in relation to extractive microdistribution. In: 34th annual meeting, the international research group on wood preservation, Stockholm, SwedenGoogle Scholar
  26. Singh P, Sulaiman O, Hashim R, Rupani P, Peng LC (2010) Biopulping of lignocellulosic material using different fungal species: a review. Rev Environ Sci Biotechnol 9(2):141–151CrossRefGoogle Scholar
  27. Tekere M, Mswaka A, Zvauya R, Read J (2001) Growth, dye degradation and ligninolytic activity studies on Zimbabwean white rot fungi. Enzyme Microb Technol 28:420–426CrossRefGoogle Scholar
  28. Tien M, Kirk T (1988) Lignin peroxidase of Phanerochaete chrysosporium. Methods Enzymol 161:238–249CrossRefGoogle Scholar
  29. Wainwright M (1992) An introduction to fungal biotechnology. Wiley, ChichesterGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • C. Y. Liew
    • 1
  • A. Husaini
    • 2
    Email author
  • H. Hussain
    • 2
  • S. Muid
    • 3
  • K. C. Liew
    • 4
  • H. A. Roslan
    • 2
  1. 1.Post Harvest Technology DivisionAgriculture Research CentreKuching, SarawakMalaysia
  2. 2.Department of Molecular Biology, Faculty of Resource Science and TechnologyUniversiti Malaysia SarawakKota Samarahan, SarawakMalaysia
  3. 3.Department of Plant Science and Environmental Ecology, Faculty of Resource Science and TechnologyUniversiti Malaysia SarawakKota Samarahan, SarawakMalaysia
  4. 4.School of International Tropical ForestryUniversiti Malaysia SabahKota Kinabalu, SabahMalaysia

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