Curdlan-like exopolysaccharide production by Cellulomonas flavigena UNP3 during growth on hydrocarbon substrates

  • Sushma Deepthi Arli
  • U. B. Trivedi
  • K. C. Patel
Original Paper

Abstract

Cellulomonas flavigena UNP3, a natural isolate from vegetable oil contaminated soil sample has been studied for growth associated exopolysaccharide (EPS) production during growth on glucose, groundnut oil and naphthalene. The EPS showed matrix formation surrounding the cells during scanning electron microscopy. Cell surface hydrophobicity and emulsifying activity studies confirmed the role of EPS as bioemulsifier. Emulsifying activity was found to increase with time (0.2 U/mg for 10 min to 0.27 U/mg for 30 min). Emulsification index, E24 value increased with the increase in EPS concentration. Degradation of polyaromatic hydrocarbons was confirmed using gas chromatography analysis. FTIR analysis showed presence of characteristic absorbance at 895.10 cm−1 for β-configuration of glucan. NMR studies also revealed EPS produced by C. flavigena UNP3 as a linear β-1, 3-d-glucan, and a curdlan like polysaccharide.

Keywords

Biodegradation Bioemulsifier Cellulomonas flavigena UNP3 Cell surface hydrophobicity Curdlan Exopolysaccharide Fourier transform infrared spectroscopy Nuclear magnetic resonance spectroscopy 

Notes

Acknowledgments

The authors are thankful to the University Grant Commission, Government of India for financial help. For SEM and spectroscopic analysis help from Sophisticated Instrumentation Centre for Applied Research and Testing (SICART), Vallabh Vidyanagar is duly acknowledged.

Supplementary material

11274_2010_593_MOESM1_ESM.doc (113 kb)
Supplementary material 1 (DOC 113 kb)

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Sushma Deepthi Arli
    • 1
  • U. B. Trivedi
    • 1
  • K. C. Patel
    • 1
  1. 1.BRD School of BiosciencesSardar Patel UniversityVallabh VidyanagarIndia

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