World Journal of Microbiology and Biotechnology

, Volume 27, Issue 5, pp 1099–1108

Occurrence of Vibrio cholerae non-O1 in three wastewater treatment plants in Agadir (Morocco)

  • Rkia Eddabra
  • Wardi Moussaoui
  • Gilles Prévost
  • François Delalande
  • Alain Van Dorsselaer
  • Olivier Meunier
  • Jean-Michel Scheftel
  • Rachida Mimouni
Original Paper


A total of 21 isolates of Vibriocholerae non-O1 strains were isolated from three wastewater treatment plants in Agadir, Morocco. The isolates were analyzed by biochemical analysis, antibiogram, pulsed-field gel electrophoresis and the MALDI-TOF patterns of their protein masses were compared. Over 67% of isolates were susceptible to antimicrobial agents tested and 14% proved resistant to both trimethoprim-sulfamethoxazole and nalidixic acid. Typing by pulsed-field gel electrophoresis with NotI digestion revealed that the V. cholerae non-O1 strains from Agadir (Morocco) have a lower level of genetic homogeneity, the restriction patterns of whole-chromosomal DNA grouped the V. cholerae O1 and V. alginolyticus strains into a separate cluster from V. metschnikovii and V. cholerae non-O1 isolates. Furthermore, to gain additional analytical accuracy and reliability in the analysis we used dendrogram based on MALDI-TOF spectral patterns generated by the BioTyper 1.1™ software. All m/z signatures of all strains tested indicate that the mass spectral data contained sufficient information to distinguish between strains of V. cholerae.


MALDI-TOF Pulsed-field gel electrophoresis Vibrio cholerae Wastewater 


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Rkia Eddabra
    • 1
    • 2
  • Wardi Moussaoui
    • 2
  • Gilles Prévost
    • 2
  • François Delalande
    • 3
  • Alain Van Dorsselaer
    • 5
  • Olivier Meunier
    • 4
  • Jean-Michel Scheftel
    • 2
  • Rachida Mimouni
    • 1
  1. 1.Laboratoire des Systèmes Aquatiques: Milieu Marin et Continental, Equipe: Ecotoxicologie et Microbiologie Appliquée, Faculté des SciencesUniversité Ibn ZohrAgadirMorocco
  2. 2.UPRES EA-4438, Physiopathologie et Médecine Translationnelle, Laboratoire de Bactériologie de la Faculté de MédecineUniversité Louis PasteurHôpitaux Universitaires de StrasbourgFrance
  3. 3.INSERM U682, Développement et Physiopathologie de l’Intestin et du PancréasUniversité de StrasbourgStrasbourgFrance
  4. 4.Laboratoire d’Hygiène HospitalièreHôpitaux Universitaires de StrasbourgStrasbourgFrance
  5. 5.Laboratoire de Spectrométrie de Masse BioOrganique, IPHC-DSA, ULP, CNRSUMR7178StrasbourgFrance

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