Endophytic fungi assemblages from 10 Dendrobium medicinal plants (Orchidaceae)

  • Juan Chen
  • Ke-Xing Hu
  • Xiao-Qiang Hou
  • Shun-Xing Guo
Original Paper

Abstract

Dendrobium is the largest genus of tropical epiphytic orchid, some of which are traditional Chinese medicinal plants. The therapeutic components varied significantly among species. Endophytic microbes (fungi) hidden in medicinal plants may play an important effect on the overall quality of herb. Investigation of fungal composition in host plants is the first step toward elucidating the relationship endophyte-therapeutic content of herbal medicine. In this study, 401 culturable fungal endophytes were isolated and identified from 10 species of medicinal Dendrobium based on morphological and molecular techniques. The results showed that endophytic fungi from Dendrobium plants exhibited high biodiversity (37 genera, about 80 species). Acremonium, Alternaria, Ampelomyces, Bionectria, Cladosporium, Colletotrichum, Fusarium, Verticillium and Xylaria were the dominant fungal endophytes. Tropical epiphytic orchids appear to vary in degree of host specificity in their endophytic fungi.

Keywords

Orchidaceae Endophyte Taxonomy Medicinal plant 

Notes

Acknowledgments

This investigation was supported by the National High Technology Research and Development Program of China (2008AA09Z405), National S&T Major Special Project on Major New Drug Innovation (2009ZX09301-003-3-3) and the National Natural Science Foundation of China (30900004). We thank Dr. Liang-Dong Guo, Institute of Microbiology, Chinese Academy of Sciences, for providing references of fungi identification.

Conflict of interest

None.

References

  1. Barnett HL, Hunter BB (1998) Illustrated genera of imperfect fungi. APS Press, St. Paul, Minnesota, pp 1–218Google Scholar
  2. Bayman P, Lebrón LL, Tremblay RL, Lodge DJ (1997) Variation in endophytic fungi from roots and leaves of Lepanthes (Orchidaceae). New Phytol 135:143–149CrossRefGoogle Scholar
  3. Brundrett MC (2006) Understanding the roles of multifunctional mycorrhizal and endophytic fungi. In: Schulz B, Boyle C, Sieber T (eds) Microbial root endophyte. Springer-Verlag, Berlin, pp 281–293CrossRefGoogle Scholar
  4. Chen XM, Guo SX (2005) Effects of four species of endophytic fungi on the growth and polysaccharide and alkaloid contents of Dendrobium nobile. China Journal of Chinese materia medica 30:253–257Google Scholar
  5. Chen SC, Tsi ZH, Luo YB (1999) Native orchids of China in colour. Science Press, Beijing, pp 1–416Google Scholar
  6. Chen XM, Yang JS, Guo SX (2000) The sterol constituens of Mycena dendrobii. Acta pharmaceutica sinica 35:367–369Google Scholar
  7. Ellis MB (1976) More dematiaceous Hyphomycetes. Commonwealth Mycological Institute, Kew, p 507Google Scholar
  8. Kumar S, Tamura K, Nei M (2004) MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163CrossRefGoogle Scholar
  9. Lavarack B, Harris W, Stocker G (2000) Dendrobium and its relatives. Kangaroo Press, New South WalesGoogle Scholar
  10. Leake JR (1994) Tansley review No. 69: the biology of myco-heterotrophic (‘saprophytic’) plants. New Phytol 127:171–216CrossRefGoogle Scholar
  11. Li SL, Han QB, Qiao CF, Song JZ, Cheng CL, Xu HX (2008) Chemical markers for the quality control of herbal medicines: an overview. Chin Med 3:7–23CrossRefGoogle Scholar
  12. Li Y, Wang CL, Guo SX, Wang YJ, Yang JS, Chen XM, Xiao PG (2009) Three new bibenzyl derivatives from Dendrobium candidum. Chem Pharm Bull 57:218–219CrossRefGoogle Scholar
  13. Nelson PE, Toussoun TA, Marasas WFO (1983) Fusarium species: an illustrated manual for identification. The Pennsylcania State University Press, University Park, pp 1–193Google Scholar
  14. Pereira OL, Kasuya MCM, Borges AC, de Araujo EF (2005) Morphological and molecular characterization of mycorrhizal fungi isolated from neotropical orchids in Brazil. Can J Bot 83:54–65CrossRefGoogle Scholar
  15. Pharmacopoeia Committee of the P. R. China (2005) Pharmacopoeia of the People’s Republic of China, vol 1. People’s Medical Publishing House, BeijingGoogle Scholar
  16. Pielou EC (1975) Ecological diversity. John Wiley, New York, p 165Google Scholar
  17. Rodriguez RJ, Henson J, Van Volkenburgh E, Hoy M, Wright L, Beckwith F, Kim Y, Redman RS (2008) Stress tolerance in plants via habitat-adapted symbiosis. ISME J 2:404–416CrossRefGoogle Scholar
  18. Sánchez MS, Bills GF, Zabalgogeazcoa I (2008) Diversity and structure of the fungal endophytic assemblages from two sympatric coastal grasses. Fungal Divers 33:87–100Google Scholar
  19. Stone JK, Bacon CW, White JF (2000) An overview of endophytic microbes: endophytism defined. In: Bacon CW, White JF (eds) Microbial endophytes. Marcel Dekker, New York, pp 3–29Google Scholar
  20. Suárez JP, Weiß M, Abele A, Garnica S, Oberwinkler F, Kottke I (2006) Diverse tulasnelloid fungi form mycorrhizas with epiphytic orchids in an Andean cloud forest. Mycol Res 110:1257–1270CrossRefGoogle Scholar
  21. Suárez JP, Weiß M, Abele A, Oberwinkler F, Kottke I (2008) Members of Sebacinales subgroup B form mycorrhizae with epiphytic orchids in a neotropical mountain rain forest. Mycol Prog 7:75–85CrossRefGoogle Scholar
  22. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599CrossRefGoogle Scholar
  23. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882CrossRefGoogle Scholar
  24. White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR Protocols: a guide to methods and applications Academic Press, New York, pp 315–322Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Juan Chen
    • 1
  • Ke-Xing Hu
    • 1
  • Xiao-Qiang Hou
    • 1
  • Shun-Xing Guo
    • 1
  1. 1.Institute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingPeople’s Republic of China

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