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Enzymes from spent mushroom substrate of Pleurotus sajor-caju for the decolourisation and detoxification of textile dyes

Abstract

The potential of ligninolytic enzymes, including lignin peroxidase (LiP) as the main enzyme from the spent mushroom substrate of Pleurotus sajor-caju was evaluated for the decolourisation of five dyes from azo and anthraquinone dye groups. Among the azo dyes, reactive black 5 and reactive orange 16 were 84.0 and 80.9% decolourised respectively, after 4 h of incubation with 45 U of LiP as compared to 32.1% decolourisation of disperse blue 79. Among the anthraquinone dyes, disperse red 60 was decolourised to 47.2% after 4 h of incubation with 45 U of LiP as compared to 5.9% decolourisation of disperse blue 56. Increasing the LiP concentration and incubation time had a positive effect on the decolourisation of anthraquinone dyes as compared to azo dyes. A 67.9% decolourisation of synthetic textile waste-water was achieved after 4 h of incubation with 25 U of LiP. Increasing the incubation time significantly increased (P < 0.05) the decolourisation of synthetic textile waste-water. Further, there was a 52.4% reduction in the toxicity of synthetic textile waste-water treated with 55 U of LiP for 4 h. However, only 35.7% reduction in toxicity was achieved when the synthetic textile waste-water was treated with 55 U of LiP for 24 h. In this study, it was shown that the spent mushroom substrate of P. sajor-caju could be a cheap source of ligninolytic enzymes for the decolourisation of dyes in textile industry wastewaters.

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Acknowledgments

The authors thank Ministry of Science, Technology and Environment, Malaysia, for the IRPA (Intensive Research in Priority Areas) grants 09-02-03-0675, 01-02-03-1002, University of Malaya for the short-term research fund (Vot F) F0145/2003A and Mr. John Kuan from Highland and Lowland Mushroom Industry, Semenyih, Selangor, Malaysia for providing Pleurotus sajor-caju bags.

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Correspondence to Sabaratnam Vikineswary.

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Singh, A.D., Vikineswary, S., Abdullah, N. et al. Enzymes from spent mushroom substrate of Pleurotus sajor-caju for the decolourisation and detoxification of textile dyes. World J Microbiol Biotechnol 27, 535–545 (2011). https://doi.org/10.1007/s11274-010-0487-3

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Keywords

  • Textile dye decolourisation
  • Spent mushroom substrate
  • Pleurotus sajor-caju
  • Toxicity reduction
  • Artemia salina