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Enzymes from spent mushroom substrate of Pleurotus sajor-caju for the decolourisation and detoxification of textile dyes


The potential of ligninolytic enzymes, including lignin peroxidase (LiP) as the main enzyme from the spent mushroom substrate of Pleurotus sajor-caju was evaluated for the decolourisation of five dyes from azo and anthraquinone dye groups. Among the azo dyes, reactive black 5 and reactive orange 16 were 84.0 and 80.9% decolourised respectively, after 4 h of incubation with 45 U of LiP as compared to 32.1% decolourisation of disperse blue 79. Among the anthraquinone dyes, disperse red 60 was decolourised to 47.2% after 4 h of incubation with 45 U of LiP as compared to 5.9% decolourisation of disperse blue 56. Increasing the LiP concentration and incubation time had a positive effect on the decolourisation of anthraquinone dyes as compared to azo dyes. A 67.9% decolourisation of synthetic textile waste-water was achieved after 4 h of incubation with 25 U of LiP. Increasing the incubation time significantly increased (P < 0.05) the decolourisation of synthetic textile waste-water. Further, there was a 52.4% reduction in the toxicity of synthetic textile waste-water treated with 55 U of LiP for 4 h. However, only 35.7% reduction in toxicity was achieved when the synthetic textile waste-water was treated with 55 U of LiP for 24 h. In this study, it was shown that the spent mushroom substrate of P. sajor-caju could be a cheap source of ligninolytic enzymes for the decolourisation of dyes in textile industry wastewaters.

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The authors thank Ministry of Science, Technology and Environment, Malaysia, for the IRPA (Intensive Research in Priority Areas) grants 09-02-03-0675, 01-02-03-1002, University of Malaya for the short-term research fund (Vot F) F0145/2003A and Mr. John Kuan from Highland and Lowland Mushroom Industry, Semenyih, Selangor, Malaysia for providing Pleurotus sajor-caju bags.

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Correspondence to Sabaratnam Vikineswary.

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Singh, A.D., Vikineswary, S., Abdullah, N. et al. Enzymes from spent mushroom substrate of Pleurotus sajor-caju for the decolourisation and detoxification of textile dyes. World J Microbiol Biotechnol 27, 535–545 (2011).

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  • Textile dye decolourisation
  • Spent mushroom substrate
  • Pleurotus sajor-caju
  • Toxicity reduction
  • Artemia salina