World Journal of Microbiology and Biotechnology

, Volume 27, Issue 2, pp 333–340 | Cite as

Phylogenetic and physiological characterization of mesophilic and thermophilic bacteria from a sewage sludge composting process in Sapporo, Japan

  • Akihiro OhnishiEmail author
  • Akihiro Nagano
  • Naoshi Fujimoto
  • Masaharu Suzuki
Original Paper


The phylogenetic and physiological characteristics of mesophilic and thermophilic bacteria isolated from a field-scale sewage sludge composter were determined by 16S rDNA and phenotype analyses. Of the 34 mesophilic isolates, 5 (15%), 16 (47%), and 3 (9%) displayed amylase, protease, and lipase activities, respectively. Among these isolates, the following species were identified based on their 16S rRNA gene sequences: Aneurinibacillus aneurinilyticus, Bacillus fortis, Bacillus subtilis, Brachybacterium paraconglomeratum, Brevibacterium otitidis, Dietzia maris, Pseudomonas xiamenensis, Staphylococcus lentus, Thermobifida fusca, Ureibacillus thermosphaericus, and Vagococcus lutrae. However, 15 isolates could not be identified as known taxa, thus indicating new bacterial taxa. Of these new taxa, it is likely that NoID A plays an important role in organic matter decomposition during composting based on its physiological characteristics. Sapporo sewage sludge compost contains a microbial ecosystem with novel bacterial biodiversity, comprising a high percentage of previously unrecognized species. This study improves our knowledge of the unique bacteria in sewage sludge compost, providing a future resource for bacterial genetic information and bacterial species of agricultural benefit.


Sewage sludge Compost Mesophilic bacteria Thermophilic bacteria 16S rRNA gene Biolog system 



We thank Morio Ishikawa, Yasuko Ohba, and Xiao-yu Huang (Faculty of Applied Bio-Science, Tokyo University of Agriculture) for their technical assistance with the sequence analysis.


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Akihiro Ohnishi
    • 1
    Email author
  • Akihiro Nagano
    • 2
  • Naoshi Fujimoto
    • 1
  • Masaharu Suzuki
    • 1
  1. 1.Department of Fermentation Science, Faculty of Applied Bio-ScienceTokyo University of AgricultureTokyoJapan
  2. 2.Technical Environmental Systems DivisionSanki Engineering Co., Ltd.TokyoJapan

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