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Development and application of a loop-mediated isothermal amplification method on rapid detection of Pseudomonas aeruginosa strains


We developed and evaluated the specificity and sensitivity of a simple loop-mediated isothermal amplification (LAMP) method for rapid detection of P. aeruginosa strains. The optimal reaction condition was found to be 65°C for 45 min, with the detection limit as 100 fg DNA/tube and 10 CFU/reaction. Application of LAMP assays were performed 426 clinical samples (including 252 P. aeruginosa and 174 non- P. aeruginosa isolates) using a rapid procedure and easy result confirmation. Sensitivity of LAMP and PCR assays was found to be 97.6% (246/252) and 90.5% (228/252), respectively; with a 100% specificity for both assays.

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This work was supported by Science Foundation of Ministry of Education of China (706046), National Natural Science Foundation of China (20436020) and State Scholarship Fund of China Scholarship Council (2008615044).

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Correspondence to Zhenbo Xu or Xiaowei He.

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Xihong Zhao and Li Wang contributed equally to this work.

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Zhao, X., Wang, L., Li, Y. et al. Development and application of a loop-mediated isothermal amplification method on rapid detection of Pseudomonas aeruginosa strains. World J Microbiol Biotechnol 27, 181–184 (2011).

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  • Loop-mediated isothermal amplification (LAMP)
  • P. aeruginosa
  • Rapid detection