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Development and application of a loop-mediated isothermal amplification method on rapid detection of Pseudomonas aeruginosa strains

Abstract

We developed and evaluated the specificity and sensitivity of a simple loop-mediated isothermal amplification (LAMP) method for rapid detection of P. aeruginosa strains. The optimal reaction condition was found to be 65°C for 45 min, with the detection limit as 100 fg DNA/tube and 10 CFU/reaction. Application of LAMP assays were performed 426 clinical samples (including 252 P. aeruginosa and 174 non- P. aeruginosa isolates) using a rapid procedure and easy result confirmation. Sensitivity of LAMP and PCR assays was found to be 97.6% (246/252) and 90.5% (228/252), respectively; with a 100% specificity for both assays.

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Acknowledgments

This work was supported by Science Foundation of Ministry of Education of China (706046), National Natural Science Foundation of China (20436020) and State Scholarship Fund of China Scholarship Council (2008615044).

Author information

Correspondence to Zhenbo Xu or Xiaowei He.

Additional information

Xihong Zhao and Li Wang contributed equally to this work.

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Zhao, X., Wang, L., Li, Y. et al. Development and application of a loop-mediated isothermal amplification method on rapid detection of Pseudomonas aeruginosa strains. World J Microbiol Biotechnol 27, 181–184 (2011). https://doi.org/10.1007/s11274-010-0429-0

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Keywords

  • Loop-mediated isothermal amplification (LAMP)
  • P. aeruginosa
  • Rapid detection