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Members of Gammaproteobacteria and Bacilli represent the culturable diversity of chitinolytic bacteria in chitin-enriched soils

Abstract

Culturable chitinolytic bacterial diversity was studied in chitin-rich soils collected from two industries involved in chitin production. A total of 27 chitinolytic isolates were isolated among which only 10 showed zone of clearance ≥4 mm on colloidal chitin agar plate. Using morphological, biochemical and 16S rDNA analysis, isolates were identified as Bacillus, Paenibacillus, Stenotrophomonas and Pseudomonas. Molecular phylogenetic analysis revealed that Gammaproteobacteria and Bacilli were found to be the predominant classes in these chitin-enriched soils. Chitinolytic bacterial population densities were significantly high and showed a rather simple community composition dominated by genus Bacillus and Stenotrophomonas (74%). This is the first report on assessing the chitinolytic bacterial diversity of soils from industries involved in chitin production.

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Acknowledgments

We gratefully acknowledge the financial support under University Grants Commission-Centre for Advanced Studies (UGC-CAS), Department of Biotechnology-Centre for Research and Education in Biology and Biotechnology (DBT-CREBB) program to the School of Life Sciences and Department of Science and Technology-Fund for Improvement of Science and Technology Infrastructure in Universities and Higher Educational Institutions (DST-FIST) programs to the Department of Plant Sciences, University of Hyderabad, and Prof. Ch. Venkata Ramana for his guidance in molecular characterization of bacterial isolates. SND and PVSRN thank DST-Centre for Nanoscience and DBT, respectively for research fellowships. We thank Dr. Domnique Gillet and Dr. Prasuna K. from Mahatani Chitosan and S. M. Agritech, respectively for providing the chitin-rich soil samples from their factory premises.

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Correspondence to Appa Rao Podile.

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Das, S.N., Sarma, P.V.S.R.N., Neeraja, C. et al. Members of Gammaproteobacteria and Bacilli represent the culturable diversity of chitinolytic bacteria in chitin-enriched soils. World J Microbiol Biotechnol 26, 1875–1881 (2010). https://doi.org/10.1007/s11274-010-0369-8

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Keywords

  • Chitin-enriched soil
  • Culturable diversity
  • Chitinolytic bacteria
  • 16S rDNA
  • Gammaproteobacteria
  • Bacilli