World Journal of Microbiology and Biotechnology

, Volume 26, Issue 8, pp 1473–1482

Molecular detection of potentially toxic cyanobacteria and their associated bacteria in lake water column and sediment

  • Konstantinos Ar. Kormas
  • Elisabeth Vardaka
  • Maria Moustaka-Gouni
  • Vasiliki Kontoyanni
  • Evi Petridou
  • Spyros Gkelis
  • Christos Neofitou
Original Paper

Abstract

We investigated the molecular diversity of cyanobacteria and bacteria during a water bloom in a lake with a long history of toxic cyanobacterial blooms (Lake Kastoria, Greece). We also tested the hypothesis whether bloom-forming cyanobacteria are preserved in the lake’s sediment 2 years after the bloom. The dominant cyanobacteria during the bloom included the potentially toxin-producing Microcystis aeruginosa and several other Chroococcales forms closely related to the genus Microcystis. This suggests that the use of cyanobacterial-specific primers seems to be very informative in describing the cyanobacteria during the water blooms. The bacterial community showed high diversity, consisting mostly of singleton and doubleton phylotypes. The majority of the phylotypes were typical lake bacteria including some potential pathogens and toxin metabolising bacteria, suggesting that the dominant toxic cyanobacteria did not have any significant effect on the bacterial community structure. In the sediment, 2 years after the water bloom, no bloom-forming cyanobacteria were retrieved, suggesting that they cannot be preserved in the sediment. Similar to the water column, sediment bacterial diversity was also high, consisting mostly of yet-uncultured bacteria that are related to environments where organic matter degradation takes place.

Keywords

Cyanobacteria Bacteria 16S rRNA Water column Sediment Lake Kastoria 

Supplementary material

11274_2010_322_MOESM1_ESM.doc (222 kb)
(DOC 221 kb)

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Konstantinos Ar. Kormas
    • 1
  • Elisabeth Vardaka
    • 2
  • Maria Moustaka-Gouni
    • 3
  • Vasiliki Kontoyanni
    • 1
  • Evi Petridou
    • 1
  • Spyros Gkelis
    • 1
    • 3
  • Christos Neofitou
    • 1
  1. 1.Department of Ichthyology and Aquatic EnvironmentUniversity of ThessalyNea IoniaGreece
  2. 2.Department of Fisheries and Aquaculture TechnologyAlexander Technological Educational Institute of ThessalonikiNew MoudaniaGreece
  3. 3.Department of BotanyAristotle University of ThessalonikiThessalonikiGreece

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