Cladosporium sphaerospermum as a new plant growth-promoting endophyte from the roots of Glycine max (L.) Merr.
- 669 Downloads
Endophytic fungi are plant symbionts that produce a variety of beneficial metabolites for plant growth and protection against herbivory and pathogens. Fourteen fungal samples were isolated from the roots of soybean cultivar Daemangkong and screened on waito-c rice for their plant growth-promoting capacity. Twelve of the fungal isolates promoted plant growth, while two inhibited it. The fungal isolate DK-1-1 induced maximum plant growth in both waito-c rice and soybean. The plant growth promotion capacity of DK-1-1 was higher than the wild type Gibberella fujikuroi. Gibberellin (GA) analysis of culture filtrate of DK-1-1 showed the presence of higher amounts of bioactive GA3, GA4, and GA7 (6.62, 2.1 and 1.26 ng/mL, respectively) along with physiologically inactive GA5, GA15, GA19, and GA24. Phylogenetic analysis of 18S rDNA sequence identified the fungal isolate as a new strain of Cladosporium sphaerospermum. Gibberellin production and plant growth-promoting ability of genus Cladosporium are reported for the first time in the present study.
KeywordsCladosporium sphaerospermum Gibberellins Plant growth Endophytic fungi 18S rDNA Waito-c rice
This study was supported by the technology development program for Agriculture and Forestry, Ministry for Agriculture, Forestry and Fisheries, Republic of Korea.
- Badillet G, de Bievre C, Spizajzen S (1982) Isolement de dematiees a partir d’ongles et de squames. Bull Soc Fr Mycologie 11:69–72Google Scholar
- Carroll GC (1986) The biology of endophytism in plants with particular reference to woody perennials. In: Fokkema NJ, Van Den Heuvel J (eds) Microbiology of the Phyllosphere. Cambridge University Press, Cambridge UK, pp 205–222Google Scholar
- Choi WY, Rim SO, Lee JH et al (2005) Isolation of gibberellins producing fungi from the root of several Sesamum indicum plants. J Microbiol Biotechnol 15(1):22–28Google Scholar
- Cohen JD, Bausher MG, Bialek K et al (1987) Comparison of a commercial ELISA assay for Indole-3-acetic acid at several stages of purification and analysis by gas chromatography-selected ion monitoring-mass spectrometry using a 3C6-labeled internal standard. Plant Physiol 84:982–986. doi: 10.1104/pp.84.4.982 CrossRefGoogle Scholar
- Franck C, Lammertyn J, Nicolaï B (2005) Metabolic profiling using GC-MS to study biochemical changes during long-term storage of pears. Proceedings of the 5th International Postharvest Symposium. F. Mencarelli and P. Tonutti. (Eds) Acta Hortic 682:1991–1998Google Scholar
- Hasan HAH (2002) Gibberellin and auxin production plant root fungi and their biosynthesis under salinity-calcium interaction. Rostlinna Vyroba 48(3):101–106Google Scholar
- Nishijima T, Koshioka M, Yamazaki H et al (1995) Endogenous gibberellins and bolting in cultivars of Japanese radish. Acta Hortic 394:199–206Google Scholar
- Park HG, Managbanag JR, Stamenova EK et al (2004) Comparative analysis of common indoor Cladosporium species based on molecular data and conidial characters. Mycotaxon 89:441–451Google Scholar
- Pereira PT, de Carvalho MM, Girio FM et al (2002) Diversity of microfungi in the phylloplane of plants growing in a Mediterranean ecosystem. J Basic Microbiol 42:396–407. doi: 10.1002/1521-4028(200212)42:6<396::AID-JOBM396>3.0.CO;2-L CrossRefGoogle Scholar
- Rim SO, Lee JH, Choi WY et al (2006) Fusarium proliferatum KGL0401 as a new gibberellin-producing fungus. J Microbiol Biotechnol 15(4):809–814Google Scholar
- Tamura K, Dudley J, Nei M et al (2007) MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol pp.1596–1599Google Scholar
- Tejesvi MV, Kini KR, Prakash HS et al (2007) Genetic diversity and antifungal activity of species of Pestalotiopsis isolated as endophytes from medicinal plants. Fungal Divers 24:37–54Google Scholar
- Tudzunski B (1997) Fungal phytohormones in pathogenic and mutualistic associations. In: Esser K, Lemke PA (eds) The mycota vol. V, plant relationship, Part A. Springer-Verlag, Berlin, pp 167–184Google Scholar
- Vazquez MM, Cesar S, Azcon R et al (2000) Interaction between arbuscular mycorrhizal fungi and other microbial inoculants (Azospirillum, Pseudomonas, Trichoderma) and their effects on microbial population and enzyme activities in the rhizosphere of maize plants. Appl Soil Ecol 15:261–272. doi: 10.1016/S0929-1393(00)00075-5 CrossRefGoogle Scholar