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Isolation of fungi and optimization of process parameters for decolorization of distillery mill effluent


Five different fungi isolated from distillery mill site in which two isolates (DF3 and DF4) had higher capabilities to remove color were identified as Emericella nidulans var. lata and Neurospora intermedia, respectively. Optimization of process parameter for decolorization was initially performed to select growth factors which were further substantiated by Taguchi approach in which seven factors, %carbon, %nitrogen, duration, pH, temperature, stirring and inoculum size, at two levels applying L-8 orthogonal array were taken for both fungi. Maximum color was removed at pH 3, temperature 30°C, stirring 125 rpm, dextrose (0.05%) and sodium nitrate (0.025%) by both fungi. After optimization, there was two-fold increase in color removal from 38 to 62% (DF3) and 31 to 64% (DF4) indicating significance of Taguchi approach in decolorization of distillery mill effluent. The mechanism for decolorization was determined by enzyme analysis, laccase and glucose oxidase, and indicated significant activity in DF3 as compared to DF4.

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This paper was supported by the research grants of University for potential of Excellence. The author (GK) thanks University Grants Commission, for providing Junior Research Fellowship Government of India, New Delhi, India. We thank Modi distilleries, Modinagar, Uttar Pradesh, India for providing effluent and sludge during the course of investigation. We thank R. K. Roy (Nutek Inc., USA) for providing free demo version of their Software Qulitek-4®.

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Correspondence to Indu Shekhar Thakur.

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Kaushik, G., Thakur, I.S. Isolation of fungi and optimization of process parameters for decolorization of distillery mill effluent. World J Microbiol Biotechnol 25, 955 (2009). https://doi.org/10.1007/s11274-009-9970-0

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  • Decolorization
  • Distillery spent wash
  • Emericella nidulans var. lata
  • Neurospora intermedia
  • Taguchi approach