Skip to main content
Log in

Optimisation of extracellular tannase production from Paecilomyces variotii

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

The tannase production by Paecilomyces variotii was confirmed by high performance thin layer chromatography (HPTLC), and substrate specificity of the tannase was determined by zymogram analysis in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS–PAGE). A clear band of activity observed after electrophoresis of culture filtrate in non-denaturing gels indicated the production of extracellular tannase by P. varoitii. HPTLC analysis revealed that gallic acid was the enzymatic degradation product of tannic acid during the fermentation process. The optimum condition for tannase production was at 72 h of incubation in shaking condition and addition of 1.5% tannic acid, 1% glucose and 0.2% sodium nitrate at temperature of 35°C and pH of 5–7. The production of extracellular tannase from Paecilomyces variotii was investigated under optimized conditions in solid-state fermentation (SSF), submerged fermentation (SmF) and liquid surface fermentation (LSF) processes. The maximum extracellular tannase production was obtained within 60 h of incubation under SSF followed by SmF and LSF.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Agbo F, Spradlin JH (1995) Enzymatic clarification of tea extracts. US Patent No 5,445,836

  • Aguilar CN, Auger C, Torres EF, Gonzalez GV (2001) Induction and repression patterns of fungal tannase in solid state and submerged cultures. Process Biochem 36:565–570

    Article  CAS  Google Scholar 

  • Ajay Kumar R, Gunasekaran P, Lakshmanan M (1999) Biodegradation of tannic acid by Citrobacter freundii isolated from a tannery effluent. J Basic Microbiol 39:161–169

    Article  CAS  Google Scholar 

  • Aoki K, Tanaka T, Shinke RL, Nishira H (1979) Detection of tannase in polyacrylamide gels. J Chromatogra 170:446–448

    Article  CAS  Google Scholar 

  • Batra A, Saxena KR (2005) Potential tannase producers from the genera Aspergillus and Penicillium. Process Biochem 40:1553–1557

    Article  CAS  Google Scholar 

  • Booth C (1971) Methods in microbiology, vol 4. Academic Press, New York, p 795

    Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Bradoo S, Gupta R, Saxena SK (1997) Parametric optimization and biochemical regulation of extracellular tannase from Aspergillus japonicus. Process Biochem 32:135–139

    Article  CAS  Google Scholar 

  • Chatterjee R, Dutta A, Banarjee R, Bhattacharyya BC (1995) Production of tannase by solid state fermentation. Bioprocess Eng 14:159–162

    Article  Google Scholar 

  • Hadi TA, Banerjee R, Bhattacharyya BC (1994) Optimisation of tannase biosynthesis by a newly isolated Rhizopus oryzae. Bioprocess Eng 11:239–243

    Article  CAS  Google Scholar 

  • Jeffries T, Choi WS, Kirk TK (1981) Nutritional regulation of lignin degradation by Phenorochaete chrysosporium. Appl Environ Microbiol 42:290–296

    CAS  Google Scholar 

  • Kar B, Banerjee R (2000) Biosynthesis of tannin acyl hydrolase from tannin-rich forest residue under different fermentation conditions. J Ind Microbiol Biotechnol 25:29–38

    Article  CAS  Google Scholar 

  • Laemmli UK (1971) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–684

    Article  Google Scholar 

  • Lekha PK, Lonsane BK (1994) Comparative titres, location and properties of tannin-acly-hydrolase produced by Aspergillus niger PKL 104 in solid state, liquid surface and submerged fermentations. Process Biochem 29:497–503

    Article  CAS  Google Scholar 

  • Lekha PK, Lonsane BK (1997) Production and application of tannin-acyl hydrolase. State of the art. Adv Appl Microbiol 44:215–260

    Article  CAS  Google Scholar 

  • Mahendran B, Raman N, Kim DJ (2006) Purification and characterization of tannase from Paecilomyces variotii: hydrolysis of tannic acid using immobilized tannase. Appl Microbiol Biotechnol 70:444–450

    Article  CAS  Google Scholar 

  • Mondal KC, Pati BR (2000) Studies on the extracellular tannase from newly isolated Bacillus licheniformis KBR 6. J Basic Microbiol 40:223–232

    Article  CAS  Google Scholar 

  • Mudgett RE (1986) Solid-state fermentation. In: Demain AL, Solomon NA (eds) Manual of industrial microbiology and biotechnology. American Society of Microbiology, Washington DC, pp 66–83

    Google Scholar 

  • Nelson KE, Pell AN, Schofiel P, Zinder S (1995) Isolation and characterization of an anaerobic ruminal bacterium capable of degrading hydrolyzable tannins. Appl Environ Microbiol 61:3293–3298

    CAS  Google Scholar 

  • Rajkumar SG, Nandy SC (1983) Isolation, purification and some properties of Penicillium chrysogenum tannase. Appl Environ Microbiol 46:525–527

    Google Scholar 

  • Sangeetha PT, Ramesh MN, Prapulla SG (2004) Production of fructosyl transferase by Aspergillus oryzae CFR 202 in solid-state fermentation using agricultural by-products. Appl Microbiol Biotechnol 65:530–537

    Article  CAS  Google Scholar 

  • Yamada H, Adachi O, Watanabe M, Sato N (1968) Studies of fungal tannase. Part I. Formation, purification and catalytic properties of tannase of Aspergillus flavus. Agri Biol Chem 32:1070–1078

    CAS  Google Scholar 

Download references

Acknowledgments

Thanks are due to Prof. N. Anand, Director, CAS in Botany, University of Madras for facilities and Tamilnadu Pollution Control Board for partial financial support.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to N. Raaman.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Raaman, N., Mahendran, B., Jaganathan, C. et al. Optimisation of extracellular tannase production from Paecilomyces variotii . World J Microbiol Biotechnol 26, 1033–1039 (2010). https://doi.org/10.1007/s11274-009-0266-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-009-0266-1

Keywords

Navigation