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Molecular identification of Staphylococcus xylosus MAK2, a new α-l-rhamnosidase producer

  • Munish Puri
  • Aneet Kaur
Original Paper

Abstract

A bacterial strain, MAK-2, was isolated as a producer of α-l-rhamnosidase from a soil sample of Dehradoon, India. The strain was identified based on morphology, physiological tests and 16S rDNA analysis. The phylogenetic analysis based on the 16S rDNA sequence, identified the isolate as Staphylococcus xylosus, a non-pathogenic member of CNS (coagulase-negative staphylococci) family. The strain was capable of producing α-l-rhamnosidase by hydrolysing flavonoids thus confirming potential application in the citrus-processing industry.

Keywords

α-l-rhamnosidase 16S rDNA sequence Staphylococcus xylosus Naringin 

Notes

Acknowledgments

Authors thank Dr. Rakesh Sharma, IGIB for providing help in 16S rDNA gene sequencing. MP thanks Council of Industrial and Scientific Research (CSIR), India for a project grant CSIR 38(1133)/07/EMR-II.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Centre for Biotechnology and Interdisciplinary Sciences (Biodeakin), Institute for Technology Research and Innovation (ITRI)Deakin UniversityMelbourneAustralia
  2. 2.Fermentation and Protein Biotechnology Laboratory, Department of BiotechnologyPunjabi UniversityPatialaIndia

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