Volatile organic compounds produced by Saccharomyces cerevisiae inhibit the in vitro development of Guignardia citricarpa, the causal agent of citrus black spot

  • Mauricio Batista Fialho
  • Leonardo Toffano
  • Marcio Pozzobon Pedroso
  • Fabio Augusto
  • Sérgio Florentino Pascholati
Original Paper

Abstract

Due to the low chemical control effectiveness of citrus black spot, caused by the fungus Guignardia citricarpa at postharvest, and to the search for alternative control methods, this study aimed to evaluate the in vitro effect of volatile organic compounds (VOCs), produced by yeast Saccharomyces cerevisiae, on G. citricarpa. It was observed that the yeast strains evaluated acted as antagonists by VOC production, whose maximum inhibitory capacity was as high as 87.2%. The presence of fermentable carbon sources in the medium was essential for the bioactive VOC production by the yeast. The analysis of VOCs produced in PDA medium by SPME–GC–MS indicated the presence of high quantities of alcohols as well as esters. An artificial VOC mixture prepared on the basis of the composition of the VOCs mimicked the inhibitory effects of the natural VOCs released by S. cerevisiae. Thus, the VOCs produced by the yeast or the artificial mixtures can be a promising control method for citrus black spot or others postharvest diseases.

Keywords

VOC Microbial interaction Inhibition Alternative control Phyllosticta Citrus 

Notes

Acknowledgments

The authors thank Prof. Luiz Carlos Basso, Ph.D., Biological Sciences Department (“Luiz de Queiroz” Agriculture School, University of São Paulo, Piracicaba, SP, Brazil), who provided the S. cerevisiae strains. This research was supported by CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico—National Council for Scientific and Technological Development), a Brazilian foundation associated to the Ministry of Science and Technology (MCT).

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Mauricio Batista Fialho
    • 1
  • Leonardo Toffano
    • 1
  • Marcio Pozzobon Pedroso
    • 2
  • Fabio Augusto
    • 2
  • Sérgio Florentino Pascholati
    • 1
  1. 1.Departamento de Nematologia e Fitopatologia, Escola Superior de Agricultura “Luiz de Queiroz”Universidade de São PauloPiracicabaBrazil
  2. 2.Departamento de Química Analítica, Instituto de QuímicaUniversidade Estadual de CampinasCampinasBrazil

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